Linn Y C, Prakash P, Ong Y W
Department of Haematology, Singapore General Hospital.
Ann Acad Med Singap. 1995 Mar;24(2):305-11.
This paper studies the prevalence of platelet antibody in multiply-transfused leukaemia and other transfusion-dependent non-leukaemic patients, using a solid phase red cell adherence (SPRCA) assay and a microlymphocytotoxicity (LCT) test. A positivity rate of 26% in the leukaemic patients and 72% in non-leukaemic patients was obtained with the SPRCA assay, compared to a higher positivity rate of 69% and 83% in the respective groups using the LCT test. Several explanations are proposed. The lower sensitivity of the SPRCA assay suggests that the LCT test may need to be run concurrently for higher diagnostic yield. This paper also compares the performance between an in-house SPRCA test method and the commercial Capture P test kit, and good concordance was shown in repeated occasions.
本文采用固相红细胞吸附（SPRCA）试验和微量淋巴细胞毒性（LCT）试验，研究多次输血的白血病患者及其他依赖输血的非白血病患者中血小板抗体的流行情况。采用SPRCA试验时，白血病患者的阳性率为26%，非白血病患者为72%；而采用LCT试验时，相应组别的阳性率分别为69%和83%，后者更高。文中提出了几种解释。SPRCA试验较低的敏感性表明，可能需要同时进行LCT试验以提高诊断率。本文还比较了内部SPRCA检测方法与商业捕获P检测试剂盒的性能，多次检测显示两者具有良好的一致性。
