Fabbro D, Tell G, Pellizzari L, Leonardi A, Pucillo C, Lonigro R, Damante G
Dipartimento di Scienze e Tecnologie Biomediche, Università di Udine, Italy.
Biochem Biophys Res Commun. 1995 Aug 24;213(3):781-8. doi: 10.1006/bbrc.1995.2198.
The homeodomain of the thyroid transcription factor-1 (TTF-1HD) shows a peculiar DNA-binding specificity, preferentially recognizing sequences having the 5'-CAAG-3' core motif. In order to detail the DNA-binding specificity of this protein, a TTF-1HD-Sepharose column chromatography was used. A sequential selection and amplification of sequences was performed. TTF-1HD binding activity for selected and unselected sequences was measured. The presence of the 5'-CAAG-3' core motif was necessary, but not sufficient, to obtain the maximal binding activity for TTF-1HD. However, several of the selected sequences do not contain the 5'-CAAG-3' core motif and are bound by TTF-1HD only 2-fold less with respect to sequences bound with the highest affinity. Therefore, these data indicate that TTF-1HD specifically recognizes a spectrum of sequences wider than previously determined.
甲状腺转录因子-1的同源结构域（TTF-1HD）表现出独特的DNA结合特异性，优先识别具有5'-CAAG-3'核心基序的序列。为了详细研究该蛋白的DNA结合特异性，使用了TTF-1HD-琼脂糖柱色谱法。对序列进行了连续的选择和扩增。测量了TTF-1HD对选定和未选定序列的结合活性。5'-CAAG-3'核心基序的存在对于获得TTF-1HD的最大结合活性是必要的，但不是充分的。然而，一些选定的序列不包含5'-CAAG-3'核心基序，并且与TTF-1HD的结合仅比与具有最高亲和力结合的序列少2倍。因此，这些数据表明TTF-1HD特异性识别的序列谱比先前确定的更宽。
