Abbracchio M P, Ceruti S, Barbieri D, Franceschi C, Malorni W, Biondo L, Burnstock G, Cattabeni F
Inst. Pharmacol. Sci. Univ. Milan, Italy.
Biochem Biophys Res Commun. 1995 Aug 24;213(3):908-15. doi: 10.1006/bbrc.1995.2215.
2-chloro-adenosine induced apoptosis of astroglial cells in rat brain cultures, as shown by flow cytometry and morphological analysis. The adenosine analogue was far more potent than several previously characterized sugars (including 2-deoxy-D-ribose and D-ribose, the sugar moiety of 2-chloro-adenosine), which trigger apoptosis in a variety of cell-lines [8-10], suggesting that the effects of 2-chloro-adenosine are only partially dictated by its sugar moiety. Nevertheless, 2-chloro-adenosine and 2-deoxy-D-ribose attenuated each other's cell death when used in combination, suggesting the involvement of common intracellular mechanisms. It is suggested that 2-chloro-adenosine may induce apoptosis via a yet-to-be identified adenosine receptor, which may have intriguing implications for both nervous system development and brain response to trauma and ischemia.
流式细胞术和形态学分析表明，2-氯腺苷可诱导大鼠脑培养物中的星形胶质细胞凋亡。腺苷类似物比几种先前表征的糖类（包括2-脱氧-D-核糖和D-核糖，2-氯腺苷的糖部分）的效力要强得多，这些糖类可在多种细胞系中引发凋亡[8-10]，这表明2-氯腺苷的作用仅部分由其糖部分决定。然而，2-氯腺苷和2-脱氧-D-核糖联合使用时可相互减弱细胞死亡，这表明存在共同的细胞内机制。有人提出，2-氯腺苷可能通过一种尚未确定的腺苷受体诱导凋亡，这可能对神经系统发育以及大脑对创伤和缺血的反应具有有趣的意义。
