Tomono M, Crilly K S, Kiss Z
Hormel Institute, University of Minnesota, Austin 55912, USA.
Biochem Biophys Res Commun. 1995 Aug 24;213(3):980-5. doi: 10.1006/bbrc.1995.2225.
In NIH 3T3 fibroblasts insulin is a much less potent mitogen than platelet-derived growth factor or fibroblast growth factor. Here we demonstrate that addition of choline phosphate (1 mM) or ethanolamine (1 mM) to these fibroblasts for a prolonged (16 h) period specifically and greatly enhanced, particularly when added in combination, the stimulatory effects of both insulin and insulin-like growth factor I on DNA synthesis. The results suggest that increased production of choline phosphate and ethanolamine, often observed in tumors and carcinogen-treated cells, may promote the mitogenic activity of insulin and insulin-like growth factor I.
在NIH 3T3成纤维细胞中，胰岛素作为有丝分裂原的效力远低于血小板衍生生长因子或成纤维细胞生长因子。在此我们证明，向这些成纤维细胞中添加磷酸胆碱（1 mM）或乙醇胺（1 mM）并持续较长时间（16小时），会特异性地大幅增强胰岛素和胰岛素样生长因子I对DNA合成的刺激作用，二者联合添加时效果尤为显著。结果表明，在肿瘤和经致癌物处理的细胞中经常观察到的磷酸胆碱和乙醇胺产量增加，可能会促进胰岛素和胰岛素样生长因子I的有丝分裂活性。
