Häcker G, Vaux D L
Walter and Eliza Hall Institute of Medical Research, Post Office Royal Melbourne Hospital, Victoria, Australia.
Biochem Cell Biol. 1994 Nov-Dec;72(11-12):451-4. doi: 10.1139/o94-060.
We have cloned mouse candidate cell death genes RP-2 and nedd2 and used Northern blot analysis to study their expression in a growth-factor-dependent cell line (FDC-P1) that can be induced to undergo apoptosis by growth factor withdrawal and in a thymoma line (ST4) that undergoes apoptosis when irradiated. RP-2 was expressed in FDC-P1 cells even when not undergoing apoptosis, and mRNA levels did not increase when apoptosis was induced by growth factor withdrawal. FDC-P1 cells expressed two genes that are similar to the Caenorhabditis elegans cell death gene ced-3: a 3.7 kilobase (kb) nedd2 message and low levels of a 5-kb message for interleukin-1 beta converting enzyme. Levels of these messages did not change with the induction of cell death, but transfection of fibroblasts with constructs expressing nedd2 caused them to undergo apoptosis. These results suggest that expression of RP-2 and nedd2 is not sufficient for apoptosis, and if the products of these genes are involved in apoptosis of FDC-P1 cells, they are regulated by posttranslational mechanisms.
我们已经克隆了小鼠候选细胞死亡基因RP-2和nedd2，并使用Northern印迹分析来研究它们在依赖生长因子的细胞系（FDC-P1）中的表达。该细胞系在生长因子撤除时可被诱导发生凋亡，以及在受照射时发生凋亡的胸腺瘤细胞系（ST4）中的表达。RP-2即使在FDC-P1细胞未发生凋亡时也有表达，当生长因子撤除诱导凋亡时，mRNA水平并未升高。FDC-P1细胞表达了两个与秀丽隐杆线虫细胞死亡基因ced-3相似的基因：一个3.7千碱基（kb）的nedd2信息和低水平的一个5 kb的白细胞介素-1β转换酶信息。这些信息的水平不会随着细胞死亡的诱导而改变，但用表达nedd2的构建体转染成纤维细胞会导致它们发生凋亡。这些结果表明，RP-2和nedd2的表达不足以导致凋亡，并且如果这些基因的产物参与FDC-P1细胞的凋亡，它们是通过翻译后机制进行调控的。
