Xi X, Sun J, Jiang S
PLA General Hospital, Beijing.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 1995;30(2):67-9.
The effects of ACh, ATP and high K+ on intracellular calcium concentration Ca2+ in outer hair cells (OHC) of the guinea pig cochleae were observed with the use of Ca2+ sensitive dye Fluo-3 and laser scanning confocal microscope. Ca2+ increase induced by these chemicals had individual temporal and spatial features. Moreover, high K+ solutions induced contraction and bending of OHC too. Taking advantage of the "optical sectioning" facility of confocal microscopy, morphography of Fluorescein-labelled living OHC and PI-dyed nuclei was reconstructed three-dimensionally. The application of confocal microscopy in OHC physiological and morphological research was discussed.
利用钙敏感染料Fluo-3和激光扫描共聚焦显微镜，观察了乙酰胆碱（ACh）、三磷酸腺苷（ATP）和高钾对豚鼠耳蜗外毛细胞（OHC）细胞内钙浓度Ca2+的影响。这些化学物质诱导的Ca2+增加具有各自的时间和空间特征。此外，高钾溶液也可诱导OHC收缩和弯曲。利用共聚焦显微镜的“光学切片”功能，对荧光素标记的活OHC和碘化丙啶（PI）染色的细胞核进行了三维形态重建。并讨论了共聚焦显微镜在OHC生理和形态学研究中的应用。
