Li Y, Huang Y, Luo S
Zhujang Hospital, First Military Medical University, Guangzhou.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 1995;30(4):227-9.
Stained with fluorescence, the intracellular free calcium concentration (Ca2+) of the cochlear out hair cell (OHC) isolated from guinea pigs was measured using ACAS 570. Streptomycin caused a remarkable rise in Ca2+ and reached peak value in 60s, decreased gradually after lasting for 60s in that value. The Ca2+ fell back to primary level in another 100s and followed by a slight drop. The distribution of free calcium in the OHC was showed. The rise of intracellular free calcium in the OHC was assumed to be a period in the ototoxicity caused by aminoglycoside antibiotics.
用荧光染色后，使用ACAS 570测量从豚鼠分离出的耳蜗外毛细胞（OHC）的细胞内游离钙浓度（Ca2+）。链霉素导致Ca2+显著升高，并在60秒时达到峰值，在该值持续60秒后逐渐下降。Ca2+在另外100秒内回落至初始水平，随后略有下降。显示了OHC中游离钙的分布。OHC中细胞内游离钙的升高被认为是氨基糖苷类抗生素耳毒性作用的一个阶段。
