用于测量隐形眼镜表面脂质沉积的荧光测定法。

Fluorescence assay for measuring lipid deposits on contact lens surfaces.

作者信息

Ho C H, Hlady V

机构信息

Center for Biopolymers at Interfaces, Department of Bioengineering, University of Utah, Salt Lake City 84112, USA.

出版信息

Biomaterials. 1995 Apr;16(6):479-82. doi: 10.1016/0142-9612(95)98821-u.

DOI:10.1016/0142-9612(95)98821-u

PMID:7654875

Abstract

A simple fluorescence assay for estimating the propensity of the contact lens surface towards the uptake of lipids and formation of lipid deposits has been developed. The assay is based on the incubation of the contact lenses with an artificial tear-lipid mixture, staining of the lenses with a fluorescent lipid probe, Nile Red, and subsequent macroscopic imaging of the fluorescent lipid deposits on the contact lens surface with a thermoelectrically cooled charge-coupled device (CCD) camera. The results of the assay show that statistically significant differences in the formation of lipid deposits exist between the surfaces of different contact lens materials.

摘要

已开发出一种简单的荧光测定法，用于评估隐形眼镜表面摄取脂质和形成脂质沉积物的倾向。该测定法基于将隐形眼镜与人工泪液-脂质混合物孵育，用荧光脂质探针尼罗红对镜片进行染色，随后用热电冷却电荷耦合器件（CCD）相机对隐形眼镜表面的荧光脂质沉积物进行宏观成像。测定结果表明，不同隐形眼镜材料表面在脂质沉积物形成方面存在统计学上的显著差异。