Matsumoto Y, Hayashi T, Hayakawa Y, Shinbo M, Niiya K, Sakuragawa N
Department of Clinical Laboratory Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
Blood Coagul Fibrinolysis. 1995 May;6(3):233-8. doi: 10.1097/00001721-199505000-00006.
This study investigated the effect of carbazochrome sodium sulphonate (AC-17) on the accumulation of tissue-type plasminogen activator antigen (t-PA:Ag) and plasminogen activator inhibitor-1 antigen (PAI-1:Ag) in culture medium of human umbilical vein endothelial cells (HUVEC). HUVEC were cultured in the presence of AC-17, and the concentration of t-PA:Ag and PAI-1:Ag in the medium was measured by an enzyme-linked immunoassay. AC-17, after 48 and 72 h incubation, significantly decreased the t-PA:Ag in the culture medium, while it had no significant effect on the PAI-1:Ag. The result of fibrin zymography was consistent with the result obtained by ELISA. It also revealed that all of the t-PA present in the culture medium formed complexes with PAI-1, indicating that AC-17 did not interfere with t-PA/PAI-1 complex formation. Furthermore, AC-17 did not inhibit the activities of t-PA and plasmin when measured by a fibrin plate method. These results suggest that AC-17 may modulate the fibrinolytic balance of blood through changing the function of endothelial cells, a new aspect of action of AC-17 as a haemostatic drug.
本研究调查了卡巴克络磺钠（AC - 17）对人脐静脉内皮细胞（HUVEC）培养基中组织型纤溶酶原激活物抗原（t - PA:Ag）和纤溶酶原激活物抑制剂 - 1抗原（PAI - 1:Ag）积累的影响。将HUVEC在AC - 17存在的情况下进行培养，并用酶联免疫吸附测定法测量培养基中t - PA:Ag和PAI - 1:Ag的浓度。在孵育48小时和72小时后，AC - 17显著降低了培养基中的t - PA:Ag，而对PAI - 1:Ag没有显著影响。纤维蛋白酶谱分析结果与酶联免疫吸附测定法获得的结果一致。该结果还表明，培养基中存在的所有t - PA均与PAI - 1形成复合物，这表明AC - 17不会干扰t - PA/PAI - 1复合物的形成。此外，通过纤维蛋白平板法测量时，AC - 17不会抑制t - PA和纤溶酶的活性。这些结果表明，AC - 17可能通过改变内皮细胞的功能来调节血液的纤溶平衡，这是AC - 17作为止血药物作用的一个新方面。
