Inamoto Y, Hiraga Y, Hanai T, Kinosita T
Teikoku Seiyaku Co., Ltd., Kagawa, Japan.
Biomed Chromatogr. 1995 May-Jun;9(3):146-9. doi: 10.1002/bmc.1130090307.
A sensitive liquid chromatographic analysis for myo-inositol was developed using glycocyamine as the post-labelling reagent. The sensitivity was 500 pmol/injection. The system was applied to analyse myo-inositol in sera from eight patients with chronic renal failure. The average concentration of serum myo-inositol was 498.6 +/- 257.0 mumol/L before haemodialysis, and 244.0 +/- 131.1 mumol/L after haemodialysis. These results indicated that the kidney is the main site of myo-inositol metabolism.
采用胍基乙胺作为柱后衍生试剂，建立了一种灵敏的液相色谱法分析肌醇。灵敏度为500 pmol/进样量。该系统用于分析8例慢性肾衰竭患者血清中的肌醇。血液透析前血清肌醇的平均浓度为498.6±257.0 μmol/L，血液透析后为244.0±131.1 μmol/L。这些结果表明，肾脏是肌醇代谢的主要场所。
