Einspahr J, Alberts D, Xie T, Ritchie J, Earnest D, Hixson L, Powell M, Roe D, Grogan T
Arizona Cancer Center, Tucson, USA.
Cancer Epidemiol Biomarkers Prev. 1995 Jun;4(4):359-66.
Measurement of proliferation rates by the more standard in vitro uptake techniques of [3H]thymidine and 5'-bromo-2'-deoxyuridine (BrdUrd) labeling indices (LIs) were compared to proliferating cell nuclear antigen (PCNA) in rectal mucosal biopsies from 16 subjects with resected colorectal cancer and 14 normal age-matched controls. Correlation coefficients for BrdUrd versus PCNA, [3H]thymidine versus PCNA, and BrdUrd versus [3H]thymidine were 0.691, 0.876, and 0.770, respectively. No significant differences (P > 0.05) were detected in total mean LIs between the LI methods for the normal group. In contrast, total PCNA LIs were found to be significantly different in the resected cancer patients when compared to either BrdUrd (P = 0.005) or [3H]thymidine (P < 0.001). A significant difference (P = 0.010) in total PCNA LI but not in total [3H]thymidine or BrdUrd LIs was also observed between normal controls and resected colorectal cancer subjects. Compartmental analysis of the cancer group versus the normals showed a significant difference in compartments 1 and 3 for PCNA LIs only. The reproducibility of two PCNA LI counts was excellent (r = 0.9). In addition, the reliability of mean LIs were > 0.8 with the exception of [3H]thymidine in the normal group (0.7). These study results demonstrate that PCNA LIs in human rectal mucosal biopsies are correlated highly with other more commonly used cellular proliferation measurements; however, PCNA LIs were found to be significantly higher than the other two methods in the resected colorectal cancer subjects.
通过[3H]胸苷和5'-溴-2'-脱氧尿苷（BrdUrd）标记指数（LIs）等更标准的体外摄取技术测量增殖率，并与16例接受过结直肠癌切除术的受试者和14例年龄匹配的正常对照者的直肠黏膜活检组织中的增殖细胞核抗原（PCNA）进行比较。BrdUrd与PCNA、[3H]胸苷与PCNA以及BrdUrd与[3H]胸苷的相关系数分别为0.691、0.876和0.770。正常组中，LI方法之间的总平均LIs未检测到显著差异（P>0.05）。相比之下，在接受过癌症切除术的患者中，与BrdUrd（P=0.005）或[3H]胸苷（P<0.001）相比，总PCNA LIs存在显著差异。在正常对照者和接受过结直肠癌切除术的受试者之间，也观察到总PCNA LI存在显著差异（P=0.010），但总[3H]胸苷或BrdUrd LIs无显著差异。癌症组与正常组的区室分析显示，仅PCNA LIs在区室1和区室3存在显著差异。两次PCNA LI计数的重复性极佳（r=0.9）。此外，除正常组中的[3H]胸苷（0.7）外，平均LIs的可靠性>0.8。这些研究结果表明，人直肠黏膜活检组织中的PCNA LIs与其他更常用的细胞增殖测量方法高度相关；然而，在接受过结直肠癌切除术的受试者中，PCNA LIs显著高于其他两种方法。
