Erickson J C, Masters B A, Kelly E J, Brinster R L, Palmiter R D
Howard Hughes Medical Institute, University of Washington, Seattle 98195, USA.
Neurochem Int. 1995 Jul;27(1):35-41. doi: 10.1016/0197-0186(94)00166-r.
Transgenic mice that express human metallothionein-III (hMT-III) were generated. Human MT-III mRNA expression was prominent in brain, resulting in a 9-fold elevation of MT-III mRNA in cortex, a 3-5-fold elevation in hippocampus, thalamus, brainstem, and olfactory bulb, and a 1.4-fold elevation in cerebellum. Human MT-III protein was detected biochemically and accounted for a 3.4-fold increase in total brain MT. The concentration of zinc (but not copper) was elevated in those brain regions that expressed the most hMT-III mRNA. The histochemically reactive pool of zinc, as measured by Timm's stain or TS-Q histofluorescence, was not appreciably altered. No changes in brain weight, morphology or histology have been noted; the mice breed normally and appear to have normal behavior.
生成了表达人金属硫蛋白-III（hMT-III）的转基因小鼠。人MT-III mRNA在脑中表达显著，导致皮质中MT-III mRNA升高9倍，海马体、丘脑、脑干和嗅球中升高3至5倍，小脑中升高1.4倍。通过生化方法检测到人MT-III蛋白，其在全脑MT中增加了3.4倍。在那些表达最多hMT-III mRNA的脑区中，锌（而非铜）的浓度升高。通过Timm染色或TS-Q组织荧光测定的锌的组织化学反应池没有明显改变。未观察到脑重量、形态或组织学的变化；这些小鼠繁殖正常，行为似乎也正常。
