Hirota K
School of Health Sciences, Okayama University, Japan.
Biol Pharm Bull. 1995 Apr;18(4):481-4. doi: 10.1248/bpb.18.481.
The distribution and relative proportions of the four isomers of alpha-, beta-, gamma- and delta-biliverdin-IX were investigated using urinary samples from seven healthy adults. The biliverdins from samples were adsorbed on talc, and the adsorbed biliverdins were then soaked in a solution of methanol and concentrated sulfuric acid for derivation to the dimethyl esters. The esters were passed to a reversed-phase cartridge for clean-up, then quantitated by HPLC on a reversed-phase column. The analyses revealed that the biliverdins in 24-h urine were comprised of 6.4 +/- 2.5 nmol alpha- and 9.3 +/- 5.4 nmol beta-isomers. The HPLC analyses suggested the presence of a delta-isomer in a much smaller amount, but no gamma-isomer was detected. The identifications of alpha- and beta-isomers were performed with co-chromatography and absorption spectra.
使用来自7名健康成年人的尿液样本，对α-、β-、γ-和δ-胆红素-IX的四种异构体的分布和相对比例进行了研究。样本中的胆红素吸附在滑石粉上，然后将吸附的胆红素浸泡在甲醇和浓硫酸溶液中衍生为二甲酯。将酯类通过反相柱进行净化，然后在反相柱上通过高效液相色谱法进行定量。分析显示，24小时尿液中的胆红素由6.4±2.5 nmol的α-异构体和9.3±5.4 nmol的β-异构体组成。高效液相色谱分析表明存在少量的δ-异构体，但未检测到γ-异构体。通过共色谱法和吸收光谱对α-和β-异构体进行了鉴定。
