血蛋白和血红素在体内被破坏后胆绿素异构体的尿排泄情况。
Urinary excretion of isomers of biliverdin after destruction in vivo of haemoproteins and haemin.
作者信息
Hirota K, Yamamoto S, Itano H A
出版信息
Biochem J. 1985 Jul 15;229(2):477-83. doi: 10.1042/bj2290477.
The amount and isomeric composition of urinary biliverdin in rabbits were analysed by h.p.l.c. Physiological values were maintained after the injection of haemin. On the other hand, when haemoglobins from several mammalian species were injected into rabbits, the excretion of biliverdin-IX alpha and biliverdin-IX beta were increased 6-18-fold and 32-66-fold respectively over physiological excretion. Injection of myoglobin resulted in a 44-fold increase in excretion of the IX alpha-isomer. Coupled oxidation with ascorbate of haemoglobin and myoglobin by oxygen produced mainly the IX alpha- and IX beta-isomers from haemoglobin and the IX alpha-isomer from myoglobin. The destruction of part of the haem from injected haemoproteins by non-enzymic chemical degradation would account for the observed respective increases in the excretion of biliverdin isomers. The excretion of biliverdin isomers after the injection of phenylhydrazine into rabbits was similar to that after the injection of haemoglobin.
采用高效液相色谱法分析了家兔尿胆绿素的含量及异构体组成。注射血红素后生理值保持稳定。另一方面，当将几种哺乳动物的血红蛋白注射到家兔体内时，胆绿素 -IXα 和胆绿素 -IXβ 的排泄量分别比生理排泄量增加了 6 - 18 倍和 32 - 66 倍。注射肌红蛋白导致 IXα 异构体的排泄量增加了 44 倍。氧气与抗坏血酸对血红蛋白和肌红蛋白的偶联氧化主要产生来自血红蛋白的 IXα 和 IXβ 异构体以及来自肌红蛋白的 IXα 异构体。注射的血红蛋白中部分血红素通过非酶化学降解而被破坏，这可以解释所观察到的胆绿素异构体排泄量的相应增加。向家兔注射苯肼后胆绿素异构体的排泄情况与注射血红蛋白后的情况相似。
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