Le Tonquèze M, Salozhin K, Dueymes M, Piette J C, Kovalev V, Shoenfeld Y, Nassonov E, Youinou P
Laboratory of Immunology, Brest University Medical School Hospital, France.
Lupus. 1995 Jun;4(3):179-86. doi: 10.1177/096120339500400304.
A group of anticardiolipin antibodies (aCL) require beta 2-glycoprotein I (beta 2GPI) to recognize their target, which might be located on endothelial cells (EC) and/or platelets. Following incubation with epithelial cells, 13 of 30 lupus sera retained EC-reactive antibodies of the IgG, IgA and IgM isotypes. Associated aCL and anti-phosphatidylethanolamine antibodies were partly absorbed on eC as well as EC. The former antibodies were more efficiently removed in the presence than in the absence of the latter. The presence of beta 2GPI in the affinity-purified aCL preparations may explain their binding to EC, as this cross-reaction was abrogated by the removal of the cofactor and restored by its re-introduction. Seventy four per cent of EC were faintly stained with polyclonal or monoclonal antibody directed to the cofactor. The beta 2GPI mediated aCL binding to EC membranes could this be influential in the development of thrombosis and/or thrombocytopenia in aCL-positive patients.
一组抗心磷脂抗体（aCL）需要β2糖蛋白I（β2GPI）来识别其靶标，该靶标可能位于内皮细胞（EC）和/或血小板上。与上皮细胞孵育后，30份狼疮血清中有13份保留了IgG、IgA和IgM同种型的EC反应性抗体。相关的aCL和抗磷脂酰乙醇胺抗体部分被EC以及上皮细胞吸收。前者的抗体在存在后者的情况下比不存在时更有效地被去除。亲和纯化的aCL制剂中存在β2GPI可能解释了它们与EC的结合，因为这种交叉反应在去除辅因子后被消除，并通过重新引入辅因子而恢复。74%的EC被针对辅因子的多克隆或单克隆抗体轻微染色。β2GPI介导的aCL与EC膜的结合可能在aCL阳性患者的血栓形成和/或血小板减少症的发展中起作用。
