Velic I, Metzler M, Hege H G, Weymann J
Environmental Toxicology and Food Chemistry, University of Kaiserslautern, Germany.
J Chromatogr B Biomed Appl. 1995 Apr 7;666(1):139-47. doi: 10.1016/0378-4347(94)00552-g.
A simple and accurate HPLC procedure was developed to quantify, in a single run, all phase I and phase II [14C]antipyrine metabolites that occur in rat and dog urine. All metabolites were subjected to thermospray-LC-MS and EI-MS in order to establish their structure. The rat metabolizes antipyrine to eight major metabolites, six of which are conjugated; 1.4% of the dose was excreted unchanged, 18.9% in a free form, 30.6% as sulfates and 21.1% as glucuronides. The dog metabolizes antipyrine to four metabolites, all as sulfate (61.0% of the dose) or glucuronide conjugates (16.2% of the dose).
开发了一种简单且准确的高效液相色谱法，可在一次运行中对大鼠和犬尿液中出现的所有I相和II相[14C]安替比林代谢物进行定量分析。对所有代谢物进行热喷雾液相色谱 - 质谱联用和电子轰击质谱分析，以确定其结构。大鼠将安替比林代谢为8种主要代谢物，其中6种为结合物；1.4%的剂量以原形排出，18.9%为游离形式，30.6%为硫酸盐形式，21.1%为葡糖醛酸苷形式。犬将安替比林代谢为4种代谢物，均为硫酸盐（占剂量的61.0%）或葡糖醛酸苷结合物（占剂量的16.2%）。
