Lehmann W D, Böttcher J, Bässmann H, Schüppel R, Schiebel H M
Biomed Mass Spectrom. 1982 Nov;9(11):477-82. doi: 10.1002/bms.1200091105.
The complex pattern of conjugates in the metabolism of antipyrine in man and rat has been investigated using an off-line combination of liquid chromatography and field desorption mass spectrometry. Using this approach, field desorption mass spectrometry enables the direct identification of underivatized glucuronides and sulphates in antipyrine metabolism on the basis of the corresponding protonated or cationized molecules representing the base peaks in the field desorption mass spectra. All conjugated metabolites of antipyrine established so far--four glucuronides and three sulphates--were identified in extracts of urine after stepwise purification by column chromatography and subsequent mass spectrometric analysis.
采用液相色谱和场解吸质谱联用的离线方法，对安替比林在人和大鼠体内代谢过程中的结合物复杂模式进行了研究。通过这种方法，场解吸质谱能够根据场解吸质谱中的基峰所代表的相应质子化或阳离子化分子，直接鉴定安替比林代谢物中未衍生化的葡萄糖醛酸苷和硫酸盐。到目前为止，在通过柱色谱逐步纯化并随后进行质谱分析的尿液提取物中，已鉴定出安替比林的所有结合代谢物——四种葡萄糖醛酸苷和三种硫酸盐。
