Li L, Wang H, Zhu S
Nephrology Institute, Beijing Medical University.
Zhonghua Yi Xue Za Zhi. 1995 May;75(5):276-9, 319.
It was found in our previous studies that two Chinese herbs, Astragali and Angelica (A & A), with a high protein diet could protect the decrease of serum albumin and markedly increase the synthesis rate of protein by 18N-glycine metabolic study in nephrotic rats. A further experiment was designed to investigate the effects of A&A and high protein intake on the level of hepatic albumin's mRNA in nephrotic rats. Male S. D. rats were divided into 3 groups: high protein diet alone (39.7% HP); HP with nephrotic syndrome (HPN); and HPN with therapy (HPNT). Nephrotic syndrome in rat was induced by accelerated nephrotoxic nephritis, withfed A&A (2 g of each in 3 ml) daily for 14 days. At the 15th day, the serum and liver were collected and the level of serum albumin and mRNA were measured with biochemistry, Northern blot, dot blot hybridization and quantity in laser density screening. The level of serum albumin in HPNT was higher than that of HPN (3.7 +/- 0.1 vs 2.9 +/- 0.29, P < 0.01) with an unchanged urinary output. The level of hepatic albumin mRNA in HPNT was higher than HPN (2.56 +/- 0.24 vs 1.7 +/- 0.12, P < 0.01) and HP (2.56 +/- 0.24 vs 1.25 +/- 0.24, P < 0.01). We conclude that in a molecular biologic level A&A combined with high protein intake could increase the albumin synthesis in the liver of nephrotic rat.
我们之前的研究发现，两种中药黄芪和当归（A&A）与高蛋白饮食相结合，可保护肾病大鼠血清白蛋白水平下降，并通过18N-甘氨酸代谢研究显著提高蛋白质合成率。进一步设计实验以研究A&A和高蛋白摄入对肾病大鼠肝脏白蛋白mRNA水平的影响。雄性SD大鼠分为3组：单纯高蛋白饮食组（39.7%HP）；高蛋白饮食合并肾病综合征组（HPN）；以及高蛋白饮食合并肾病综合征且接受治疗组（HPNT）。大鼠肾病综合征通过加速肾毒性肾炎诱导，每天灌胃给予A&A（各2g溶于3ml），持续14天。在第15天，采集血清和肝脏，通过生化、Northern印迹、斑点印迹杂交和激光密度扫描定量法测定血清白蛋白水平和mRNA。HPNT组血清白蛋白水平高于HPN组（3.7±0.1对2.9±0.29，P<0.01），尿量无变化。HPNT组肝脏白蛋白mRNA水平高于HPN组（2.56±0.24对1.7±0.12，P<0.01）和HP组（2.56±0.24对1.25±0.24，P<0.01）。我们得出结论，在分子生物学水平上，A&A与高蛋白摄入相结合可增加肾病大鼠肝脏中白蛋白的合成。
