胸苷磷酸化酶活性与人类结直肠癌中白细胞介素1α、α干扰素和γ干扰素表达的相关性

The correlation of thymidine phosphorylase activity with the expression of interleukin 1 alpha, interferon alpha and interferon gamma in human colorectal carcinoma.

作者信息

Takebayashi Y, Yamada K, Ohmoto Y, Sameshima T, Miyadera K, Yamada Y, Akiyama S, Aikou T

机构信息

First Department of Surgery, Faculty of Medicine, Kagoshima University, Japan.

出版信息

Cancer Lett. 1995 Aug 16;95(1-2):57-62. doi: 10.1016/0304-3835(95)03865-t.

DOI:10.1016/0304-3835(95)03865-t

PMID:7656244

Abstract

Thymidine phosphorylase (dThdPase) is an angiogenic enzyme and seems to be related to an angiogenesis in human colorectal carcinoma. The incidence of dThdPase-positive cells was significantly correlated with microvessel count in 21 human colorectal carcinomas. Interleukin 1 alpha (IL-1 alpha), tumor necrosis factor alpha (TNF-alpha), interferon alpha (IFN-alpha) interferon gamma (IFN-gamma) induce dThdPase activity in human cancer cell lines. To study whether this phenomenon occurs in the human colorectal carcinomas, we examined the correlation between dThdPase activity and the expression levels of IL-1 alpha, TNF-alpha, IFN-alpha and IFN-gamma in colorectal carcinoma tissues. dThdPase activity was assayed by the methods of Friedkin and Robert, and the expression level of IL-1 alpha, TNF-alpha, IFN-alpha and IFN-gamma was determined by ELISA. dThdPase activity was significantly correlated with the amount of IL-1 alpha (n = 19, r = 0.347, P = 0.0001), INF-alpha (n = 18, r = 0.717, P = 0.008), and IFN-gamma (n = 4, r = 0.9777, P = 0.0234) in human colorectal carcinomas. However, the dThdPase activity was not correlated with the amount of TNF-alpha (n = 21, r = 0.235, P = 0.2682). These results suggested that the expression levels of IL-1 alpha, IFN-alpha and IFN-gamma are correlated with dThdPase activity in human colorectal carcinomas and that these cytokines may cause angiogenesis by inducing the expression of dThdPase.

摘要

胸苷磷酸化酶（dThdPase）是一种血管生成酶，似乎与人类结直肠癌的血管生成有关。在21例人类结直肠癌中，dThdPase阳性细胞的发生率与微血管计数显著相关。白细胞介素1α（IL-1α）、肿瘤坏死因子α（TNF-α）、干扰素α（IFN-α）、干扰素γ（IFN-γ）可诱导人类癌细胞系中的dThdPase活性。为研究这种现象是否在人类结直肠癌中发生，我们检测了结直肠癌组织中dThdPase活性与IL-1α、TNF-α、IFN-α和IFN-γ表达水平之间的相关性。采用Friedkin和Robert的方法测定dThdPase活性，通过酶联免疫吸附测定法（ELISA）测定IL-1α、TNF-α、IFN-α和IFN-γ的表达水平。在人类结直肠癌中，dThdPase活性与IL-1α的量（n = 19，r = 0.347，P = 0.0001）、INF-α（n = 18，r = 0.717，P = 0.008）和IFN-γ（n = 4，r = 0.9777，P = 0.0234）显著相关。然而，dThdPase活性与TNF-α的量无关（n = 21，r = 0.235，P = 0.2682）。这些结果表明，IL-1α、IFN-α和IFN-γ的表达水平与人类结直肠癌中的dThdPase活性相关，并且这些细胞因子可能通过诱导dThdPase的表达而导致血管生成。