Takebayashi Y, Yamada K, Maruyama I, Fujii R, Akiyama S, Aikou T
First Department of Surgery, Kagoshima University, Japan.
Cancer Lett. 1995 May 25;92(1):1-7. doi: 10.1016/0304-3835(94)03754-7.
Thymidine phosphorylase (dThdPase) is an enzyme involved in pyrimidine nucleoside metabolism. dThdPase activity is increased in several types of malignant tumors. Recently, we demonstrated that dThdPase is identical to platelet-derived endothelial cell growth factor (PD-ECGF) and that dThdPase has angiogenic activity. We measured dThdPase activity and the level of thrombomodulin (TM) as a marker for endothelial cells in colorectal carcinomas and adjacent normal tissues from 21 patients, and in adenomas from 13 patients. The average dThdPase activity of colorectal carcinomas (11.58 +/- 6.30 nmol/100 micrograms protein/h) was significantly higher than that of adenomas (8.57 +/- 4.14 nmol/100 micrograms protein/h) or normal tissues (4.89 +/- 3.16 nmol/100 micrograms protein/h). In immunohistochemical study, the expression of dThdPase was observed more frequently in colorectal carcinomas than in adenomas or normal mucosas. The amount of TM in colorectal carcinomas (8.32 +/- 5.07 ng/100 micrograms protein) was significantly higher than that of adenomas (4.51 +/- 4.49 ng/100 micrograms protein) or normal tissues (3.51 +/- 2.78 ng/100 micrograms protein). dThdPase activity in human colorectal carcinomas, adenomas and normal tissues was significantly correlated with the expression of TM in these tissues. These results indicate that the expression levels of both dThdPase and TM in colorectal carcinomas are higher than those in colorectal adenomas and normal tissues and suggest that dThdPase may be involved in angiogenesis in human colorectal carcinomas, adenomas and normal tissues.
胸苷磷酸化酶（dThdPase）是一种参与嘧啶核苷代谢的酶。dThdPase活性在几种类型的恶性肿瘤中升高。最近，我们证明dThdPase与血小板衍生的内皮细胞生长因子（PD-ECGF）相同，并且dThdPase具有血管生成活性。我们测量了21例患者结直肠癌及相邻正常组织以及13例患者腺瘤中dThdPase活性和作为内皮细胞标志物的血栓调节蛋白（TM）水平。结直肠癌的平均dThdPase活性（11.58±6.30 nmol/100微克蛋白质/小时）显著高于腺瘤（8.57±4.14 nmol/100微克蛋白质/小时）或正常组织（4.89±3.16 nmol/100微克蛋白质/小时）。在免疫组织化学研究中，结直肠癌中dThdPase的表达比腺瘤或正常黏膜中更频繁地观察到。结直肠癌中TM的量（8.32±5.07 ng/100微克蛋白质）显著高于腺瘤（4.51±4.49 ng/100微克蛋白质）或正常组织（3.51±2.78 ng/100微克蛋白质）。人结直肠癌、腺瘤和正常组织中的dThdPase活性与这些组织中TM的表达显著相关。这些结果表明，结直肠癌中dThdPase和TM的表达水平均高于结直肠腺瘤和正常组织，并提示dThdPase可能参与人结直肠癌、腺瘤和正常组织中的血管生成。
