Li F, Che D, Liu S
Department of Pathology, Tongji Medical University, Wuhan.
Zhonghua Bing Li Xue Za Zhi. 1995 Jun;24(3):139-42.
Northern blot, slot blot hybridization and quantitative immunocytochemistry analyses were used to study the effects of hypoxia on the expression of PDGF gene and PDGF-B chain protein in cultured pulmonary artery endothelial cells (PAEC). Hypoxia (3% oxygen) significantly increased PDGF-B chain mRNA in PAEC, whereas mRNA level of PDGF-A chain was not increased significantly in comparison with normoxic control (21% oxygen). PDGF-B chain immunoreactivity on PAEC exposed to hypoxia was markedly stronger than that of normoxic PAEC and PDGF-B China immunoreaction. was found in cytoplasm and perinuclear region of the hypoxic PAEC, however, only in the nuclear region of normoxic PAEC. Increased expression of PDGF-B chain protein roughly paralleling that of PDGF-B mRNA was also observed. These results suggest that hypoxia may stimulate the expression of PDGF-B chain by PAEC in vivo and PDGF may play an important role in the vascular remodeling of hypoxic pulmonary hypertension.
采用Northern印迹法、狭缝印迹杂交法和定量免疫细胞化学分析法，研究缺氧对培养的肺动脉内皮细胞（PAEC）中血小板衍生生长因子（PDGF）基因表达及PDGF - B链蛋白表达的影响。缺氧（3%氧气）显著增加了PAEC中PDGF - B链mRNA的表达，而与常氧对照（21%氧气）相比，PDGF - A链的mRNA水平未显著增加。暴露于缺氧环境的PAEC上的PDGF - B链免疫反应性明显强于常氧PAEC，且在缺氧PAEC的细胞质和核周区域发现了PDGF - B链免疫反应，然而，仅在常氧PAEC的核区域发现。还观察到PDGF - B链蛋白表达的增加与PDGF - B mRNA的增加大致平行。这些结果表明，缺氧可能在体内刺激PAEC表达PDGF - B链，且PDGF可能在缺氧性肺动脉高压的血管重塑中起重要作用。
