[缺氧内皮细胞条件培养基诱导的猪肺动脉平滑肌细胞血小板衍生生长因子（PDGF）mRNA表达]
[Platelet-derived growth factor (PDGF) mRNA expression of porcine pulmonary artery smooth muscle cells induced by hypoxic endothelial cells conditioned medium].
作者信息
Li F, Zhang Y, Che D
机构信息
Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang 110001.
出版信息
Zhonghua Bing Li Xue Za Zhi. 1998 Dec;27(6):425-8.
OBJECTIVE
To study the effect of hypoxic endothelial cell conditioned medium on the autocrine of PDGF in porcine pulmonary artery smooth muscle cells (PASMC).
METHODS
Experimental groups included serum-free medium group (control group), normoxic endothelial cell conditioned medium (NECCM) group and hypoxic endothelial cell conditioned medium (HECCM) group. The expression of PDGF-A and -B chain mRNA was determined by in situ hybridization and image analysis.
RESULTS
A weak expression for PDGF-A and PDGF-B mRNA was observed in PASMC cultured in serum free medium (control group); HECCM markedly enhanced the expression of PDGF-A and PDGF-B mRNA in PASMC, both being about 1.8-fold and 1.73-fold of the control group respectively, and 1.4-fold and 1.5-fold of the NECCM group respectively (P < 0.01).
CONCLUSION
PDGF may be an autocrine growth factor of PASMC induced by HECCM and may play an important role in vascular structural remodeling of hypoxic pulmonary hypertension.
目的
研究缺氧内皮细胞条件培养基对猪肺动脉平滑肌细胞（PASMC）中血小板衍生生长因子（PDGF）自分泌的影响。
方法
实验组包括无血清培养基组（对照组）、常氧内皮细胞条件培养基（NECCM）组和缺氧内皮细胞条件培养基（HECCM）组。采用原位杂交和图像分析检测PDGF-A和-B链mRNA的表达。
结果
在无血清培养基中培养的PASMC（对照组）中观察到PDGF-A和PDGF-B mRNA的弱表达；HECCM显著增强了PASMC中PDGF-A和PDGF-B mRNA的表达，分别约为对照组的1.8倍和1.73倍，分别为NECCM组的1.4倍和1.5倍（P<0.01）。
结论
PDGF可能是HECCM诱导的PASMC自分泌生长因子，可能在缺氧性肺动脉高压的血管结构重塑中起重要作用。
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