Li F, Zhang Y, Che D
Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang 110001.
Zhonghua Bing Li Xue Za Zhi. 1998 Dec;27(6):425-8.
To study the effect of hypoxic endothelial cell conditioned medium on the autocrine of PDGF in porcine pulmonary artery smooth muscle cells (PASMC).
Experimental groups included serum-free medium group (control group), normoxic endothelial cell conditioned medium (NECCM) group and hypoxic endothelial cell conditioned medium (HECCM) group. The expression of PDGF-A and -B chain mRNA was determined by in situ hybridization and image analysis.
A weak expression for PDGF-A and PDGF-B mRNA was observed in PASMC cultured in serum free medium (control group); HECCM markedly enhanced the expression of PDGF-A and PDGF-B mRNA in PASMC, both being about 1.8-fold and 1.73-fold of the control group respectively, and 1.4-fold and 1.5-fold of the NECCM group respectively (P < 0.01).
PDGF may be an autocrine growth factor of PASMC induced by HECCM and may play an important role in vascular structural remodeling of hypoxic pulmonary hypertension.
研究缺氧内皮细胞条件培养基对猪肺动脉平滑肌细胞（PASMC）中血小板衍生生长因子（PDGF）自分泌的影响。
实验组包括无血清培养基组（对照组）、常氧内皮细胞条件培养基（NECCM）组和缺氧内皮细胞条件培养基（HECCM）组。采用原位杂交和图像分析检测PDGF-A和-B链mRNA的表达。
在无血清培养基中培养的PASMC（对照组）中观察到PDGF-A和PDGF-B mRNA的弱表达；HECCM显著增强了PASMC中PDGF-A和PDGF-B mRNA的表达，分别约为对照组的1.8倍和1.73倍，分别为NECCM组的1.4倍和1.5倍（P<0.01）。
PDGF可能是HECCM诱导的PASMC自分泌生长因子，可能在缺氧性肺动脉高压的血管结构重塑中起重要作用。
