Guo Z, Zhu Y, Yan Y, Xi P
PUMC Hospital, CAMS, Beijing.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1995 Apr;17(2):104-8.
Human neutrophil and its elastase play an important role in the pathogenesis of various lung diseases. We investigated the damaging effect of human neutrophils stimulated with PMA (phorbol myristate acetate 10ng/ml) or calcium ionophase A23187 (2ng/ml) on isolated perfused rat lungs. The study specimens consisted of 4 groups of animals. A control, B perfused with human neutrophils, C perfused with human neutrophils stimulated with PMA, and D perfused with human neutrophils stimulated with A23187. By using the ELISA method, we found that the level of human neutrophil elastase (HNE) in perfusate increased significantly at 30min (1.68 +/- 0.79ng/ml P < 0.05) and 60min (2.35 +/- 0.87 ng/ml P < 0.01) after PMA stimulating, but the HNE in the lung lavage fluid of the same group only slightly increased and the concentration of protein in lavage fluid did not change. After stimulating with A23187, the concentration of HNE in perfusate increased markedly at 30min (4.03 +/- 1.96ng/ml P < 0.01) and maintained at high level (P < 0.01). There was a significant increase of HNE level in lung lavage fluid (2.49 +/- 0.61ng/ml) from group D rat lungs, compared with group A, B or C, and the protein as well (1.61 +/- 0.58mg/ml P < 0.05). All the results indicate that the stimulated human neutrophils can release elastase in perfused rat lung. Neutrophil stimulated with A23187 can increase pulmonary permeability and injure the lung. We suggest that the lung damage, caused by human neutrophils, may be the effect of neutrophil elastase or the synergistic effect with some other substances derived from neutrophil.
人类中性粒细胞及其弹性蛋白酶在多种肺部疾病的发病机制中起重要作用。我们研究了用佛波酯（10ng/ml 肉豆蔻酸佛波醇乙酯）或钙离子载体 A23187（2ng/ml）刺激的人类中性粒细胞对离体灌注大鼠肺的损伤作用。研究标本由4组动物组成。A组为对照组，B组用人类中性粒细胞灌注，C组用经佛波酯刺激的人类中性粒细胞灌注，D组用经 A23187 刺激的人类中性粒细胞灌注。通过酶联免疫吸附测定（ELISA）法，我们发现佛波酯刺激后30分钟（1.68±0.79ng/ml，P<0.05）和60分钟（2.35±0.87ng/ml，P<0.01）时，灌注液中人类中性粒细胞弹性蛋白酶（HNE）水平显著升高，但同一组肺灌洗液中的 HNE 仅略有增加，且灌洗液中蛋白质浓度未改变。用 A23187 刺激后，灌注液中 HNE 浓度在30分钟时显著升高（4.03±1.96ng/ml，P<0.01）并维持在高水平（P<0.01）。与A组、B组或C组相比，D组大鼠肺灌洗液中HNE水平显著升高（2.49±0.61ng/ml），蛋白质水平也显著升高（1.61±0.58mg/ml，P<0.05）。所有结果表明，受刺激的人类中性粒细胞可在灌注大鼠肺中释放弹性蛋白酶。经 A23187 刺激的中性粒细胞可增加肺通透性并损伤肺。我们认为，人类中性粒细胞引起的肺损伤可能是中性粒细胞弹性蛋白酶的作用，或与中性粒细胞衍生的其他某些物质的协同作用。
