Yu M, Zhang C, Shi L, Shan X
PUMC Hospital, PUMC, Beijing.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1995 Apr;17(2):151-4.
We used Enzyme Linked Immuno-Sorbent Assay (ELISA) to measure IgG RF of supernatant of cultured knee synovia in 45 patients with various rheumatic diseases (Harvest time for the supernatant was 1,4,7,14,28 days). 25 cases of patients with osteoarthritis (OA) and non-synovitis (Non-S) were regarded as the negative control. IgG RF was positive in 4 of 12 patients with rheumatoid arthritis (RA), whose IL-1 bioactivity is also high. Meanwhile these 4 patients had not received disease-modifying anti-rheumatic drugs (DMARDs) and their clinical and laboratory parameters were active. IgG RF was negative in other groups. It is suggested that humoral factor such as IgG RF and cytokine such as IL-1 may play the synergistic role in the pathogenesis of RA and DMARDs may suppress these two kinds of pathological factors.
我们采用酶联免疫吸附测定法（ELISA）检测了45例患有各种风湿性疾病患者培养的膝关节滑膜上清液中的IgG类风湿因子（RF）（上清液的采集时间为1、4、7、14、28天）。25例骨关节炎（OA）患者和无滑膜炎（Non-S）患者被视为阴性对照。12例类风湿关节炎（RA）患者中有4例IgG RF呈阳性，其IL-1生物活性也较高。同时，这4例患者未接受改善病情抗风湿药物（DMARDs）治疗，其临床和实验室参数均处于活动期。其他组的IgG RF均为阴性。提示IgG RF等体液因子和IL-1等细胞因子可能在RA发病机制中起协同作用，而DMARDs可能抑制这两种病理因素。
