Steinman L, Tzehoval E, Cohen I R, Segal S, Glickman E
Eur J Immunol. 1978 Jan;8(1):29-34. doi: 10.1002/eji.1830080107.
We have investigated the sequential interaction of macrophages, initiator T lymphocytes (ITL) and recruited T lymphocytes (RTL) in the development of a T cell-mediated response to soluble antigens. Macrophages were pulsed with soluble antigens and used to sensitize ITL in vitro. The ITL were irradiated to prevent their proliferation and then injected into the footpads of syngeneic mice. Sensitized ITL were found to recruit immunospecific RTL in the draining lymph nodes, as determined by a thymidine uptake assay of the lymph node cells in vitro. The richest source of lymphocytes with ITL activity was the thymus, and progressively less activity was detectable among spleen or lymph node lymphocytes. The magnitude of the subsequent RTL response could be modified by genetic differences between the ITL and the antigen-pulsed macrophages that were used to sensitize them. Thus, ITL conveyed an immunogenic signal to RTL whose magnitude reflected the genotype of the macrophages, but whose specificity was directed by determinants of the soluble antigen.
我们研究了巨噬细胞、起始T淋巴细胞（ITL）和募集性T淋巴细胞（RTL）在可溶性抗原介导的T细胞应答过程中的顺序性相互作用。用可溶性抗原刺激巨噬细胞，然后在体外用于使ITL致敏。对ITL进行照射以防止其增殖，然后将其注射到同基因小鼠的足垫中。通过体外对淋巴结细胞进行胸苷摄取试验确定，致敏的ITL可在引流淋巴结中募集免疫特异性RTL。具有ITL活性的淋巴细胞最丰富的来源是胸腺，在脾脏或淋巴结淋巴细胞中可检测到的活性逐渐降低。随后RTL应答的强度可因用于致敏它们的ITL与抗原刺激的巨噬细胞之间的遗传差异而改变。因此，ITL向RTL传递了一个免疫原性信号，其强度反映了巨噬细胞的基因型，但其特异性由可溶性抗原的决定簇决定。
