Nataf P, Hadjiisky P, Lechat P, Mougenot N, Peuchmaurd M, Gouezo R, Gerota J, Cabrol C, Gandjbakhch I
Centre de Recherche des Techniques Chirurgicales et Remplacements Cardio-Pulmonaires, Association Claude Bernard-Ecole de Chirurgie, Paris, France.
Cryobiology. 1995 Aug;32(4):327-33. doi: 10.1006/cryo.1995.1033.
The ability of human internal mammary artery smooth muscle cells to maintain histoenzymatic activity and contractile response after various times of cold anoxia prior to and following cryostorage was evaluated. The results showed that the enzyme histochemical status of human mammary arteries was largely unchanged after both cold anoxia and cryopreservation. Neither in fresh nor in cryopreserved mammary arteries did cold anoxia for up to 24 h change maximal contractile responses to potassium depolarization and norepinephrine. However, compared to unfrozen controls, the contractile responses were significantly reduced in cryopreserved mammary arteries. In conclusion, after cryopreservation of human mammary arteries, the enzyme activities were globally maintained, whereas the contractile responses were reduced. For up to 24 h after harvesting cold anoxia at 4 degrees C is well tolerated and allows preservation of metabolic and functional properties of these arteries.
评估了人类乳内动脉平滑肌细胞在冷冻保存前后经历不同时长冷缺氧后维持组织酶活性和收缩反应的能力。结果表明，人类乳内动脉的酶组织化学状态在冷缺氧和冷冻保存后基本未变。在新鲜和冷冻保存的乳内动脉中，长达24小时的冷缺氧均未改变对钾去极化和去甲肾上腺素的最大收缩反应。然而，与未冷冻的对照相比，冷冻保存的乳内动脉收缩反应显著降低。总之，人类乳内动脉冷冻保存后，酶活性总体得以维持，而收缩反应降低。在4℃收获后进行长达24小时的冷缺氧耐受性良好，并能保留这些动脉的代谢和功能特性。
