Reguigne-Arnould I, Couillin P, Mollicone R, Fauré S, Fletcher A, Kelly R J, Lowe J B, Oriol R
INSERM U.178, Villejuif, France.
Cytogenet Cell Genet. 1995;71(2):158-62. doi: 10.1159/000134098.
Five on the seven cloned human fucosyltransferase genes have been mapped to two clusters, one on 19q and the other on 19p. Comparative DNA sequence analysis showed the Généthon microsatellite D19S596 lies 2.2 kb downstream of the coding region of FUT1, indicating that the cluster comprising the closely linked FUT1 and FUT2 genes is located 4 cM distal to D19S412 (lod score 13.7) and 9 cM proximal to D19S571 (lod score 11.7). Polymorphic markers of FUT3, FUT5, and FUT6 were used for linkage analysis with 14 Généthon microsatellites in Indonesian families. These three loci constitute a cluster on 19p, located between the Généthon microsatellites D19S216 and D19S567, which are known to be only 1 cM distant from each other. Two cross-overs, one between FUT6 and FUT3 and the other between FUT3 and FUT5, suggest the gene order 19pter-D19S216-FUT6-FUT3-FUT5-D19S567++ +-cen. Comparison of genetic and physical maps suggests that the FUT6-FUT3-FUT5 cluster is located on 19p13.3 and the FUT1-FUT2 cluster on 19q13.3. FUT6, FUT3 and FUT5 genes share more than 85% homology and encode three similar, but distinct alpha(1,3) fucosyltransferases. FUT1 and FUT2 share about 70% homology and encode two distinct alpha(1,2)fucosyltransferases. No sequence homology was found between the genes of the two clusters. The members of each of these two clusters have probably emerged by duplication and divergent evolution of two unrelated ancestor genes.
七个克隆的人岩藻糖基转移酶基因中的五个已被定位到两个基因簇，一个在19号染色体长臂（19q）上，另一个在19号染色体短臂（19p）上。比较DNA序列分析表明，Généthon微卫星D19S596位于FUT1编码区下游2.2 kb处，这表明由紧密连锁的FUT1和FUT2基因组成的基因簇位于D19S412远端4厘摩（lod分数为13.7），D19S571近端9厘摩（lod分数为11.7）。FUT3、FUT5和FUT6的多态性标记用于与印度尼西亚家庭中的14个Généthon微卫星进行连锁分析。这三个基因座在19p上构成一个基因簇，位于Généthon微卫星D19S216和D19S567之间，已知它们彼此仅相距1厘摩。两次交换，一次在FUT6和FUT3之间，另一次在FUT3和FUT5之间，提示基因顺序为19号染色体短臂末端-D19S216-FUT6-FUT3-FUT5-D19S567++ +-着丝粒。遗传图谱和物理图谱的比较表明，FUT6-FUT3-FUT5基因簇位于19p13.3，FUT1-FUT2基因簇位于19q13.3。FUT6、FUT3和FUT5基因具有超过85%的同源性，并编码三种相似但不同的α(1,3)岩藻糖基转移酶。FUT1和FUT2具有约70%的同源性，并编码两种不同的α(1,2)岩藻糖基转移酶。在这两个基因簇的基因之间未发现序列同源性。这两个基因簇中的每一个成员可能都是由两个不相关的祖先基因通过复制和趋异进化产生的。
