McCurley R S, Recinos A, Olsen A S, Gingrich J C, Szczepaniak D, Cameron H S, Krauss R, Weston B W
Department of Pediatrics, University of North Carolina, Chapel Hill 27599-7220, USA.
Genomics. 1995 Mar 1;26(1):142-6. doi: 10.1016/0888-7543(95)80094-3.
Sialyl Lewis x and related fucosylated glycans are differentially expressed in human cells and form ligands for selectin adhesion receptors. alpha(1,3)Fucosyltransferases (FUTs) that complete their biosynthesis also show tissue specificity. We have established physical maps of the FUT3-6 loci to study regulation of this gene family. FUT4 has previously been localized to chromosome 11q21; FUT3, FUT6, and now FUT5 are localized to chromosome 19p13.3. Conventional and pulsed-field gel electrophoresis mapping of total genomic DNA and large genomic clones were used to generate a fine map of both loci, defining the order, orientation, and distances between FUTs. A P1 clone with all three 19p FUT genes in tandem orientation was isolated and used to study regions flanking FUT3, -5, and -6. Our studies provide preliminary information to study regulation of human FUT genes.
唾液酸化路易斯x及相关岩藻糖基化聚糖在人类细胞中差异表达，并形成选择素黏附受体的配体。完成其生物合成的α(1,3)岩藻糖基转移酶（FUTs）也表现出组织特异性。我们已构建了FUT3 - 6基因座的物理图谱，以研究该基因家族的调控。FUT4先前已定位到染色体11q21；FUT3、FUT6以及现在的FUT5定位到染色体19p13.3。利用总基因组DNA和大型基因组克隆的常规及脉冲场凝胶电泳图谱来生成两个基因座的精细图谱，确定FUTs之间的顺序、方向和距离。分离出一个串联排列有所有三个19p FUT基因的P1克隆，并用于研究FUT3、-5和-6侧翼区域。我们的研究为研究人类FUT基因的调控提供了初步信息。
