De Cabo S F, Santos J, Fernández-Piqueras J
Departamento de Biologiá, Facultad de Ciencias, Universidad Autoónoma de Madrid, Spain.
Cytogenet Cell Genet. 1995;71(2):187-92. doi: 10.1159/000134104.
Undermethylating agents are frequently used in the study of DNA methylation. However, the drugs of choice to induce experimental DNA undermethylation, 5-azacytidine and 5-aza-2'-deoxycytidine, are known to be cytotoxic. We report on S-adenosyl-L-homocysteine (SAH) as an alternative agent for inducing undermethylation of human DNA in vivo. The molecular and cytological evidence presented in this paper clearly suggests that SAH could be even more efficient than the cytidine analogs; in addition, SAH does not exhibit the secondary toxic effects that cripple the usefulness of cytidine analogs.
DNA 低甲基化剂常用于 DNA 甲基化研究。然而，已知用于诱导实验性 DNA 低甲基化的首选药物 5-氮杂胞苷和 5-氮杂-2'-脱氧胞苷具有细胞毒性。我们报告了 S-腺苷-L-高半胱氨酸（SAH）作为一种在体内诱导人 DNA 低甲基化的替代药物。本文提供的分子和细胞学证据清楚地表明，SAH 可能比胞苷类似物更有效；此外，SAH 不会表现出削弱胞苷类似物效用的继发性毒性作用。
