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Induction of G- and R-banding in human chromosomes by the demethylating agent S-adenosyl-L-homocysteine.

作者信息

De Cabo S F, Sentís C, Fernández-Piqueras J

机构信息

Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.

出版信息

Histochemistry. 1992 Jul;97(6):499-501. doi: 10.1007/BF00316070.

DOI:10.1007/BF00316070
PMID:1429010
Abstract

In this report we describe the procedure of growing human lymphocytes with the demethylating agent S-adenosyl-L-homocysteine (SAH). After this treatment, which is not toxic for cell survival, both R- and G-banding were obtained by new experimental procedures: R-bands have been directly demonstrated with the GC-specific fluorochrome chromomycin A3 without the necessity of any AT-specific counterstaining agent; simultaneous G-banding and active nucleolar organizer regions have been obtained by silver impregnation of chromosomes and subsequent Giemsa staining. These results suggest a possible relationship between local differences in DNA methylation and the determination of the banded chromosome structure.

摘要

相似文献

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Histochemistry. 1992 Jul;97(6):499-501. doi: 10.1007/BF00316070.
2
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引用本文的文献

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An adaptation of the Maxam and Gilbert sequencing method to the study of human metaphase chromosomes.将马克萨姆和吉尔伯特测序方法应用于人类中期染色体研究。
Histochemistry. 1994 Mar;101(3):205-7. doi: 10.1007/BF00269545.
2
S-adenosyl-L-homocysteine: a non-cytotoxic hypomethylating agent.S-腺苷-L-高半胱氨酸：一种无细胞毒性的低甲基化剂。
Experientia. 1994 Jul 15;50(7):658-9. doi: 10.1007/BF01952867.

本文引用的文献

1
Pharmacological and biochemical aspects of S-adenosylhomocysteine and S-adenosylhomocysteine hydrolase.S-腺苷同型半胱氨酸及S-腺苷同型半胱氨酸水解酶的药理学与生物化学特性
Pharmacol Rev. 1982 Sep;34(3):223-53.
2
Methylation and chromatin structure.甲基化与染色质结构。
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:577-84. doi: 10.1101/sqb.1983.047.01.068.
3
5-Azacytidine-induced undercondensations in human chromosomes.5-氮杂胞苷诱导的人类染色体凝聚不足
Hum Genet. 1984;67(3):257-63. doi: 10.1007/BF00291352.
4
Differential staining of the X-chromosome during meiosis of orthoptera by a silver impregnation procedure.通过银浸染法对直翅目减数分裂过程中的X染色体进行差异染色。
Chromosoma. 1982;85(5):707-11. doi: 10.1007/BF00330782.
5
Counterstain-enhanced chromosome banding.复染增强染色体显带
Hum Genet. 1981;57(1):1-14.
6
DNA methylation affects the formation of active chromatin.DNA甲基化影响活性染色质的形成。
Cell. 1986 Feb 28;44(4):535-43. doi: 10.1016/0092-8674(86)90263-1.
7
Evolution of chromosome bands: molecular ecology of noncoding DNA.染色体带的演变：非编码DNA的分子生态学
J Mol Evol. 1989 Jun;28(6):469-86. doi: 10.1007/BF02602928.
8
Specific protection of methylated CpGs in mammalian nuclei.哺乳动物细胞核中甲基化CpG的特异性保护。
Cell. 1989 Aug 11;58(3):509-17. doi: 10.1016/0092-8674(89)90431-5.
9
A view of interphase chromosomes.间期染色体的视图。
Science. 1990 Dec 14;250(4987):1533-40. doi: 10.1126/science.2274784.
10
Effects of sinefungin and S-adenosylhomocysteine on DNA and protein methyltransferases from Streptomyces and other bacteria.西奈芬净和S-腺苷高半胱氨酸对链霉菌及其他细菌的DNA和蛋白质甲基转移酶的影响。
FEMS Microbiol Lett. 1990 Jun 1;57(3):239-43.