Lowry P A, Deacon D M, Whitefield P, Rao S, Quesenberry M, Quesenberry P J
Division of Hematology/Oncology, University of Massachusetts Medical Center, Worcester 01655, USA.
Exp Hematol. 1995 Sep;23(10):1135-40.
Megakaryocyte progenitors are notable for their response to synergistic cytokine combinations and apparently early position in the hematopoietic differentiation pathway. Although high-proliferative-potential murine megakaryocyte progenitors have been described, they have previously been primarily observed as unilineage colonies. We describe a subclass of agar-based high-proliferative-potential colony forming cells (HPP-CFC) derived from populations enriched for early hematopoietic progenitors which, when stimulated with the combination of CSF-1, G-CSF, GM-CSF, IL-1 alpha, IL-3 and SCF, produces colonies with trilineage potential for macrophages, granulocytes, and megakaryocytes. The high proliferative potential megakaryocyte mixed (HPP-Meg-Mix) colony-forming cell is defined as a cell meeting traditional criteria for HPP-CFC but additionally containing 20 or more megakaryocytes per colony. The combination of high-proliferative-potential, multilineage differentiation, and megakaryocytic lineage capacity suggests that the HPP-Meg-Mix marks a very early hematopoietic progenitor cell.
巨核细胞祖细胞以其对协同细胞因子组合的反应以及在造血分化途径中明显较早的位置而著称。尽管已经描述了具有高增殖潜能的小鼠巨核细胞祖细胞，但此前它们主要被观察为单系集落。我们描述了一类基于琼脂的高增殖潜能集落形成细胞（HPP-CFC）的亚类，其来源于富含早期造血祖细胞的群体，当用CSF-1、G-CSF、GM-CSF、IL-1α、IL-3和SCF的组合刺激时，可产生具有巨噬细胞、粒细胞和巨核细胞三系潜能的集落。高增殖潜能巨核细胞混合（HPP-Meg-Mix）集落形成细胞被定义为符合HPP-CFC传统标准但每个集落另外含有20个或更多巨核细胞的细胞。高增殖潜能、多系分化和巨核细胞系能力的组合表明，HPP-Meg-Mix标志着一种非常早期的造血祖细胞。
