Le Calvé M, Segalen J, Quernee D, Lavault M T, Lescoat D
Laboratoire d'Histologie, d'Embryologie et de Génétique, Faculté de Médecine, Rennes, France.
Hum Reprod. 1995 May;10(5):1141-4. doi: 10.1093/oxfordjournals.humrep.a136107.
Diamine oxidase (DAO), an enzyme which degrades polyamines, is present at a very high level in human seminal plasma and is assumed to come mainly from the prostate. The possible relationships between DAO activity and biochemical markers of accessory sex glands were evaluated in 139 men in barren marriages. Four groups were formed: normozoospermic (n = 41), asthenozoospermic (n = 29), oligoasthenozoospermic (n = 35) and azoospermic (n = 34). DAO activity was the highest in the asthenozoospermic group and was significantly different from that in the azoospermic one. For all specimens, a positive correlation was demonstrated between DAO activity and the prostatic markers citric acid and acid phosphatase. However, DAO activity was correlated with citric acid only in the oligoasthenozoospermic and the azoospermic groups. Acid phosphatase and citric acid were linked in all groups. These results implicate the DAO enzyme in changes in sperm metabolism leading to a loss of motility and suggest that DAO comes partly from the upper part of the genital tract (testis and/or epididymis), in addition to the prostatic gland secretion, accounting for the absence of correlation with prostatic markers in normozoospermic and asthenozoospermic groups.
二胺氧化酶（DAO）是一种降解多胺的酶，在人类精浆中含量极高，据推测主要来自前列腺。在139名不育男性中评估了DAO活性与附属性腺生化标志物之间的可能关系。分为四组：正常精子症组（n = 41）、弱精子症组（n = 29）、少弱精子症组（n = 35）和无精子症组（n = 34）。DAO活性在弱精子症组中最高，与无精子症组有显著差异。对于所有样本，DAO活性与前列腺标志物柠檬酸和酸性磷酸酶之间呈正相关。然而，DAO活性仅在少弱精子症组和无精子症组中与柠檬酸相关。酸性磷酸酶和柠檬酸在所有组中都有关联。这些结果表明DAO酶参与了导致精子活力丧失的精子代谢变化，并表明DAO除了来自前列腺分泌外，部分还来自生殖道上部（睾丸和/或附睾），这解释了正常精子症组和弱精子症组中与前列腺标志物缺乏相关性的原因。
