DeWoody J A, Honeycutt R L, Skow L C
Department of Veterinary Anatomy and Public Health, Texas A&M University, College Station 77843, USA.
J Hered. 1995 Jul-Aug;86(4):317-9. doi: 10.1093/oxfordjournals.jhered.a111593.
A genomic library of DNA from white-tailed deer was constructed for the isolation of d(AC)n microsatellite repeats. PCR primers were designed from regions flanking each repeat and used to amplify DNA samples from a pedigreed herd of white-tailed deer and other artiodactyls. Allelic frequencies, PIC values, and heterozygosity values are reported for five polymorphic markers scored in 41 animals. Homologs of two of the five markers were assigned to bovine chromosomes 4 and 23, respectively, using a panel of bovine+hamster hybrid somatic cell lines.
构建了白尾鹿DNA的基因组文库，用于分离d(AC)n微卫星重复序列。从每个重复序列侧翼区域设计PCR引物，并用于扩增来自白尾鹿纯种群体和其他偶蹄动物的DNA样本。报告了在41只动物中评分的5个多态性标记的等位基因频率、PIC值和杂合度值。使用一组牛+仓鼠杂种体细胞系，将5个标记中的2个同源物分别定位到牛的4号和23号染色体上。
