Røed K H
Department of Morphology, Genetics and Aquatic Biology, Norwegian College of Veterinary Medicine, Oslo, Norway.
Hereditas. 1998;129(1):19-25. doi: 10.1111/j.1601-5223.1998.00019.x.
The possibility of using microsatellite primers developed in Bovidae to amplify microsatellite markers in Cervidae was surveyed by using 75 microsatellite primer sets of bovine, ovine or caprine origin to analyse DNA from moose, red deer, reindeer and roe deer from Scandinavia. On average for the four cervids, approximately 50% of the ovine/caprine primer pairs amplified a specific PCR product, compared to only 16% of the bovine primers. Approximately 50% of both ovine/caprine and bovine primers that amplified a specific product were polymorphic, giving 15 polymorphic microsatellite markers in moose, 11 in red deer, 21 in reindeer and 10 in roe deer. Reindeer had a higher proportion of polymorphic loci, more alleles per locus and higher mean heterozygosity than the other cervids.
利用牛科动物中开发的微卫星引物扩增鹿科动物微卫星标记的可能性进行了调查，使用了75套源自牛、绵羊或山羊的微卫星引物来分析来自斯堪的纳维亚半岛的驼鹿、马鹿、驯鹿和狍的DNA。对于这四种鹿科动物而言，平均约50%的绵羊/山羊引物对能扩增出特异性PCR产物，相比之下，牛的引物只有16%能扩增出产物。能扩增出特异性产物的绵羊/山羊引物和牛引物中，约50%具有多态性，在驼鹿中产生了15个多态性微卫星标记，马鹿中有11个，驯鹿中有21个，狍中有10个。与其他鹿科动物相比，驯鹿具有更高比例的多态性位点、每个位点更多的等位基因以及更高的平均杂合度。
