Menotti-Raymond M A, O'Brien S J
Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201, USA.
J Hered. 1995 Jul-Aug;86(4):319-22. doi: 10.1093/oxfordjournals.jhered.a111594.
Short tandem repeat polymorphisms (STRP), or microsatellites, are widespread among vertebrate genomes and are useful in gene mapping and population studies due to a high level of length polymorphism. We describe here the isolation, characterization, and PCR amplification of 10 microsatellite loci from the domestic cat, Felis catus. The flanking primer sequences were conserved among other Felidae species, and amplification products demonstrated abundant polymorphism in puma, lion, cheetah, and domestic cat. The cheetah sample exhibited the lowest level of polymorphism for these loci among felid species.
短串联重复多态性（STRP），即微卫星，在脊椎动物基因组中广泛存在，由于其高度的长度多态性，在基因图谱绘制和群体研究中很有用。我们在此描述了从家猫（Felis catus）中分离、鉴定和PCR扩增10个微卫星位点的过程。侧翼引物序列在其他猫科动物物种中是保守的，扩增产物在美洲狮、狮子、猎豹和家猫中表现出丰富的多态性。在猫科动物物种中，猎豹样本在这些位点上表现出最低水平的多态性。
