Department of Wildlife Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, 202002, India.
Department of Biotechnology, V.B.S. Purvanchal University, Jaunpur, Uttar Pradesh, 222003, India.
Mol Biol Rep. 2019 Jun;46(3):3025-3033. doi: 10.1007/s11033-019-04739-1. Epub 2019 Mar 12.
Information on the level and distribution of genetic variation is important for conservation plan of captive population of an endangered species such as tiger and cheetah. We assayed the frequency of microsatellites in the genomic and genic sequences of wild cats (Panthera tigris, Acinonyx jubatus) and compared it with the domestic cat (Felis catus). Frequency, relative abundance and density of microsatellites were highest in the domestic cat when compared with wild cats. The frequency of microsatellites was positively correlated with the G+C content of genomic and genic sequences. The maximum frequency of microsatellites among all three sequence sets was of di-nucleotide repeats (genomic-88.1%; genic-70.4%), whereas the hexa-nucleotide repeat represents < 0.5%. Motif conservation study among the genomic and genic sequences revealed conservation of 81.3% and 51.0% motif within the members of family Felidae. A total 40,233 primers from genic sequences were designed in order to enrich the members of family Felidae with genomic resources. The designed primers could be useful in determining the molecular genetics of population structure and individualization of a particular cat.
有关濒危物种（如老虎和猎豹）圈养种群遗传变异水平和分布的信息对于保护计划至关重要。我们检测了野生猫科动物（ Panthera tigris 、 Acinonyx jubatus ）基因组和基因序列中微卫星的频率，并将其与家猫（ Felis catus ）进行了比较。与野生猫科动物相比，家猫的微卫星频率、相对丰度和密度最高。微卫星的频率与基因组和基因序列的 G+C 含量呈正相关。在所有这三组序列中，二核苷酸重复（基因组 88.1%；基因 70.4%）的微卫星出现频率最高，而六核苷酸重复则<0.5%。对基因组和基因序列的基序保守性研究表明，猫科动物的成员中保守了 81.3%和 51.0%的基序。从基因序列设计了总共 40233 个引物，以便用基因组资源丰富猫科动物的成员。设计的引物可用于确定特定猫的种群结构的分子遗传学和个体识别。
