Izuel M, Fainboim H, Morelli A, Fainboim L, Roisman F R
Laboratorio de Inmunogenética, Hosptial de Clínicas José de San Martín, Facultad de Medicina, Buenos Aires.
Medicina (B Aires). 1994;54(6):635-40.
We have analyzed the expression of HBsAg on different subpopulations of peripheral blood cells from hepatitis B virus (HBV) infected patients using two-color immunofluorescence and flow cytometry. An average of 7.4 +/- 0.8% and 9.8 +/- 1.2% HBsAg + cells were found among cells from acute and chronic hepatitis B patients, respectively. When studied by two-color immunofluorescence, HBsAg was limited to cells expressing the B-cell restricted antigen CD19 (16.7 +/- 2.4%), whereas for the other subsets studied (CD3+, CD4+, CD8+, CD67+, CD68+) the percentage of positivity was lower than 4%. The expression of viral antigen on B cells might be relevant for a number of functional interactions between HBsAg+ B and T lymphocytes.
我们运用双色免疫荧光和流式细胞术，分析了乙型肝炎病毒（HBV）感染患者外周血细胞不同亚群上乙肝表面抗原（HBsAg）的表达情况。在急性和慢性乙型肝炎患者的细胞中，分别平均发现7.4±0.8%和9.8±1.2%的HBsAg阳性细胞。通过双色免疫荧光研究发现，HBsAg仅限于表达B细胞限制性抗原CD19的细胞（16.7±2.4%），而对于所研究的其他亚群（CD3+、CD4+、CD8+、CD67+、CD68+），阳性百分比低于4%。B细胞上病毒抗原的表达可能与HBsAg阳性B淋巴细胞和T淋巴细胞之间的多种功能相互作用有关。
