Colucci G, Colombo M, Del Ninno E, Paronetto F
Gastroenterology. 1983 Nov;85(5):1138-45.
To characterize the inflammatory infiltrate of chronic active hepatitis we have examined 26 liver biopsy specimens [16 hepatitis B surface antigen-negative and 10 hepatitis B surface antigen-positive] with monoclonal antibodies and antiimmunoglobulins that react against the following mononuclear cell populations: T cells, T4 cells, T8 cells, monocytes-macrophages, Ia-positive cells, surface Ig-bearing cells, natural killer/killer cells. A biotin-avidin-fluorescein technique was used and the number of positive cells was counted in a portal tract that was more severely involved. The T cells predominate in all the specimens. The T4:T8 cell ratio and the number of T and T4 cells in the hepatitis B surface antigen-negative patients are higher than in the hepatitis B surface antigen-positive patients. While natural killer/killer cells, surface Ig-bearing cells, and macrophages are always rare, Ia-positive cells are numerous and the double-staining technique demonstrates a fair number of cells displaying both T-cell and Ia markers. These findings, while not supporting a major role of killer cells in chronic active hepatitis, suggest a different alteration of T-cell subsets in hepatitis B surface antigen-positive and -negative chronic active hepatitis.
为了描述慢性活动性肝炎的炎性浸润情况，我们用单克隆抗体和抗免疫球蛋白检测了26份肝活检标本（16份乙型肝炎表面抗原阴性和10份乙型肝炎表面抗原阳性），这些抗体和抗免疫球蛋白可与以下单核细胞群体发生反应：T细胞、T4细胞、T8细胞、单核细胞-巨噬细胞、Ia阳性细胞、表面带有免疫球蛋白的细胞、自然杀伤/杀伤细胞。采用生物素-抗生物素蛋白-荧光素技术，在受累更严重的门管区计数阳性细胞数量。所有标本中T细胞占主导。乙型肝炎表面抗原阴性患者的T4:T8细胞比值以及T细胞和T4细胞数量高于乙型肝炎表面抗原阳性患者。虽然自然杀伤/杀伤细胞、表面带有免疫球蛋白的细胞和巨噬细胞一直很少见，但Ia阳性细胞数量众多，双重染色技术显示有相当数量的细胞同时显示T细胞和Ia标记。这些发现虽然不支持杀伤细胞在慢性活动性肝炎中起主要作用，但提示乙型肝炎表面抗原阳性和阴性的慢性活动性肝炎中T细胞亚群存在不同改变。
