García-Patrone M, Tandecarz J S
Instituto de Investigaciones Bioquímicas, Fundación Campomar, Buenos Aires, Argentina.
Mol Cell Biochem. 1995 Apr 12;145(1):29-37. doi: 10.1007/BF00925710.
Two glycoproteins (205 and 72 kDa) were found in Bacillus thuringiensis sporangia. They were predominantly localized in the exosporium and/or the spore coat, although a small proportion was also found in membranes. A method for the dissociation of hydrophobic aggregates that resist the usual conditions of SDS-PAGE is described. Using this method we established that the 205 kDa glycoprotein is a multimer of the 72 kDa one. Deglycosylation of the 205 kDa and 72 kDa glycoproteins with trifluoromethanesulfonic acid yielded a 54 kDa polypeptide in both cases. At least three species of oligosaccharides were O-glycosidically linked to serines of the 54 kDa polypeptide chain. One of the oligosaccharides had N-acetylgalactosamine at the reducing end, rhamnose and a component not yet identified.
在苏云金芽孢杆菌的芽孢囊中发现了两种糖蛋白（205 kDa和72 kDa）。它们主要定位于芽孢外壁和/或芽孢衣，不过在细胞膜中也发现了一小部分。本文描述了一种解离抵抗SDS-PAGE常规条件的疏水聚集体的方法。利用该方法我们确定205 kDa糖蛋白是72 kDa糖蛋白的多聚体。用三氟甲磺酸对205 kDa和72 kDa糖蛋白进行去糖基化处理，在两种情况下均产生了一条54 kDa的多肽。至少有三种寡糖以O-糖苷键连接到54 kDa多肽链的丝氨酸上。其中一种寡糖在还原端含有N-乙酰半乳糖胺、鼠李糖和一种尚未鉴定的成分。
