单细胞全基因组扩增：PEP和标记PCR的数学分析
Whole genome amplification of single cells: mathematical analysis of PEP and tagged PCR.
作者信息
Sun F, Arnheim N, Waterman M S
机构信息
Department of Mathetmatics, University of Southern California, Los Angeles 90089-1113, USA.
出版信息
Nucleic Acids Res. 1995 Aug 11;23(15):3034-40. doi: 10.1093/nar/23.15.3034.
Abstract
We construct a mathematical model for two whole genome amplification strategies, primer extension preamplification (PEP) and tagged polymerase chain reaction (tagged PCR). An explicit formula for the expected target yield of PEP is obtained. The distribution of the target yield and the coverage properties of these two strategies are studied by simulations. From our studies we find that polymerase with high processivity may increase the efficiency of PEP and tagged PCR.
摘要
我们构建了一个针对两种全基因组扩增策略的数学模型，即引物延伸预扩增（PEP）和标记聚合酶链反应（标记PCR）。获得了PEP预期目标产量的显式公式。通过模拟研究了这两种策略的目标产量分布和覆盖特性。从我们的研究中发现，具有高持续合成能力的聚合酶可能会提高PEP和标记PCR的效率。
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