在Mn2+存在的情况下，用DNaseI切割后抗体单链Fv序列的随机重组。 - Suppr

Random recombination of antibody single chain Fv sequences after fragmentation with DNaseI in the presence of Mn2+.

作者信息

Lorimer I A, Pastan I

机构信息

Laboratory of Molecular Biology, DCBDC, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4255, USA.

出版信息

Nucleic Acids Res. 1995 Aug 11;23(15):3067-8. doi: 10.1093/nar/23.15.3067.

DOI:10.1093/nar/23.15.3067

PMID:7659531

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307151/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce4e/307151/0af880f9092b/nar00015-0275-a.jpg

相似文献

Random recombination of antibody single chain Fv sequences after fragmentation with DNaseI in the presence of Mn2+.在Mn2+存在的情况下，用DNaseI切割后抗体单链Fv序列的随机重组。

Nucleic Acids Res. 1995 Aug 11;23(15):3067-8. doi: 10.1093/nar/23.15.3067.

Engineered single chain antibody fragments for radioimmunotherapy.用于放射免疫治疗的工程化单链抗体片段

Q J Nucl Med Mol Imaging. 2004 Dec;48(4):279-88.

Optimization of DNA shuffling for high fidelity recombination.用于高保真重组的DNA改组优化。

Nucleic Acids Res. 1997 Mar 15;25(6):1307-8. doi: 10.1093/nar/25.6.1307.

Human single-chain Fv fragments from a combinatorial library using the loxP-Cre recombination system.利用loxP-Cre重组系统从组合文库中筛选出的人单链Fv片段。

Hum Antibodies. 1999;9(1):67-77.

Broadening the impact of antibody phage display technology. Amplification of immunoglobulin sequences from species other than humans or mice.扩大抗体噬菌体展示技术的影响。扩增来自人类或小鼠以外物种的免疫球蛋白序列。

Methods Mol Biol. 2002;178:73-86.

A large semi-synthetic single-chain Fv phage display library based on chicken immunoglobulin genes.一个基于鸡免疫球蛋白基因的大型半合成单链Fv噬菌体展示文库。

BMC Biotechnol. 2004 Apr 1;4:6. doi: 10.1186/1472-6750-4-6.

Pharmacokinetics and biodistribution of genetically-engineered antibodies.基因工程抗体的药代动力学与生物分布

Q J Nucl Med. 1998 Dec;42(4):225-41.

The in vivo characteristics of genetically engineered divalent and tetravalent single-chain antibody constructs.基因工程改造的二价和四价单链抗体构建体的体内特性

Nucl Med Biol. 2005 Feb;32(2):157-64. doi: 10.1016/j.nucmedbio.2004.11.003.

Phage libraries for generation of anti-botulinum scFv antibodies.用于生成抗肉毒杆菌单链抗体片段（scFv）抗体的噬菌体文库。

Methods Mol Biol. 2000;145:219-40. doi: 10.1385/1-59259-052-7:219.

[Gene cloning, expression and immunological identification of a single chain antibody fragment 3D3 against dengue type 3 virus].[登革3型病毒单链抗体片段3D3的基因克隆、表达及免疫学鉴定]

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 1998 Sep;12(3):229-32.

引用本文的文献

Dissection of SARS Coronavirus Spike Protein into Discrete Folded Fragments.将严重急性呼吸综合征冠状病毒刺突蛋白切割成离散的折叠片段。

Tsinghua Sci Technol. 2006 Aug;11(4):490-494. doi: 10.1016/S1007-0214(06)70222-X. Epub 2006 Jul 26.

Directed evolution of a β-mannanase from to improve catalytic activity in acidic and thermophilic conditions.一种β-甘露聚糖酶的定向进化，以提高其在酸性和嗜热条件下的催化活性。

Biotechnol Biofuels. 2017 Jun 2;10:143. doi: 10.1186/s13068-017-0833-x. eCollection 2017.

An in vitro compartmentalization-based method for the selection of bond-forming enzymes from large libraries.一种基于体外区室化从大型文库中筛选形成键的酶的方法。

Biotechnol Bioeng. 2016 Aug;113(8):1647-57. doi: 10.1002/bit.25939. Epub 2016 Feb 8.

Target-specific variants of Flp recombinase mediate genome engineering reactions in mammalian cells.Flp重组酶的靶向特异性变体介导哺乳动物细胞中的基因组工程反应。

FEBS J. 2015 Sep;282(17):3323-33. doi: 10.1111/febs.13345. Epub 2015 Jul 1.

Directed evolution of the substrate specificity of dialkylglycine decarboxylase.二烷基甘氨酸脱羧酶底物特异性的定向进化

Biochim Biophys Acta. 2015 Feb;1854(2):146-55. doi: 10.1016/j.bbapap.2014.12.003. Epub 2014 Dec 10.

Library generation by gene shuffling.通过基因改组进行文库构建。

Curr Protoc Mol Biol. 2014 Jan 6;105:Unit 15.12.. doi: 10.1002/0471142727.mb1512s105.

Identification of viral peptide fragments for vaccine development.鉴定用于疫苗开发的病毒肽片段。

Methods Mol Biol. 2009;515:261-74. doi: 10.1007/978-1-59745-559-6_18.

Random dissection to select for protein split sites and its application in protein fragment complementation.用于选择蛋白质裂解位点的随机切割及其在蛋白质片段互补中的应用。

Protein Sci. 2009 Feb;18(2):399-409. doi: 10.1002/pro.42.

Single mutations convert an outward K+ channel into an inward K+ channel.单个突变可将外向钾离子通道转变为内向钾离子通道。

Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):2871-6. doi: 10.1073/pnas.0712349105. Epub 2008 Feb 19.

Evolution of variants of yeast site-specific recombinase Flp that utilize native genomic sequences as recombination target sites.利用天然基因组序列作为重组靶位点的酵母位点特异性重组酶Flp变体的进化。

Nucleic Acids Res. 2006;34(18):5259-69. doi: 10.1093/nar/gkl548. Epub 2006 Sep 26.

本文引用的文献

Affinity maturation of human growth hormone by monovalent phage display.通过单价噬菌体展示实现人生长激素的亲和力成熟

J Mol Biol. 1993 Dec 5;234(3):564-78. doi: 10.1006/jmbi.1993.1612.

Selection of beta-lactamase on filamentous bacteriophage by catalytic activity.通过催化活性在丝状噬菌体上选择β-内酰胺酶

J Mol Biol. 1994 Apr 8;237(4):415-22. doi: 10.1006/jmbi.1994.1244.

Rapid evolution of a protein in vitro by DNA shuffling.通过DNA改组在体外实现蛋白质的快速进化。

Nature. 1994 Aug 4;370(6488):389-91. doi: 10.1038/370389a0.

Applied evolution. The progeny of sexual PCR.应用进化。有性PCR的后代。

Nature. 1994 Aug 4;370(6488):324-5. doi: 10.1038/370324a0.

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution.通过随机片段化和重新组装进行DNA改组：用于分子进化的体外重组

Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10747-51. doi: 10.1073/pnas.91.22.10747.

Combinatorial multiple cassette mutagenesis creates all the permutations of mutant and wild-type sequences.组合多盒式诱变可产生突变型和野生型序列的所有排列组合。

Biotechniques. 1995 Feb;18(2):194-6.

Cloning, expression and characterization of the Fv fragments of the anti-carbohydrate mAbs B1 and B5 as single-chain immunotoxins.抗碳水化合物单克隆抗体B1和B5的Fv片段作为单链免疫毒素的克隆、表达及特性分析

Protein Eng. 1994 Dec;7(12):1509-15. doi: 10.1093/protein/7.12.1509.

Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites.盒式诱变：一种在特定位点产生多个突变的有效方法。

Gene. 1985;34(2-3):315-23. doi: 10.1016/0378-1119(85)90140-4.

B3(Fv)-PE38KDEL, a single-chain immunotoxin that causes complete regression of a human carcinoma in mice.B3(Fv)-PE38KDEL，一种能使小鼠体内的人类癌瘤完全消退的单链免疫毒素。

Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8616-20. doi: 10.1073/pnas.88.19.8616.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验

Random recombination of antibody single chain Fv sequences after fragmentation with DNaseI in the presence of Mn2+.

作者信息

机构信息

出版信息

相似文献

引用本文的文献

本文引用的文献

文献AI研究员

用中文搜PubMed

文档翻译

Suppr 超能文献

相似文献

引用本文的文献

本文引用的文献