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Random recombination of antibody single chain Fv sequences after fragmentation with DNaseI in the presence of Mn2+.

作者信息

Lorimer I A, Pastan I

机构信息

Laboratory of Molecular Biology, DCBDC, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4255, USA.

出版信息

Nucleic Acids Res. 1995 Aug 11;23(15):3067-8. doi: 10.1093/nar/23.15.3067.

DOI:10.1093/nar/23.15.3067
PMID:7659531
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307151/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce4e/307151/0af880f9092b/nar00015-0275-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce4e/307151/0af880f9092b/nar00015-0275-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce4e/307151/0af880f9092b/nar00015-0275-a.jpg

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本文引用的文献

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Affinity maturation of human growth hormone by monovalent phage display.通过单价噬菌体展示实现人生长激素的亲和力成熟
J Mol Biol. 1993 Dec 5;234(3):564-78. doi: 10.1006/jmbi.1993.1612.
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Selection of beta-lactamase on filamentous bacteriophage by catalytic activity.通过催化活性在丝状噬菌体上选择β-内酰胺酶
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Rapid evolution of a protein in vitro by DNA shuffling.通过DNA改组在体外实现蛋白质的快速进化。
一种基于体外区室化从大型文库中筛选形成键的酶的方法。
Biotechnol Bioeng. 2016 Aug;113(8):1647-57. doi: 10.1002/bit.25939. Epub 2016 Feb 8.
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Target-specific variants of Flp recombinase mediate genome engineering reactions in mammalian cells.Flp重组酶的靶向特异性变体介导哺乳动物细胞中的基因组工程反应。
FEBS J. 2015 Sep;282(17):3323-33. doi: 10.1111/febs.13345. Epub 2015 Jul 1.
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Directed evolution of the substrate specificity of dialkylglycine decarboxylase.二烷基甘氨酸脱羧酶底物特异性的定向进化
Biochim Biophys Acta. 2015 Feb;1854(2):146-55. doi: 10.1016/j.bbapap.2014.12.003. Epub 2014 Dec 10.
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Library generation by gene shuffling.通过基因改组进行文库构建。
Curr Protoc Mol Biol. 2014 Jan 6;105:Unit 15.12.. doi: 10.1002/0471142727.mb1512s105.
7
Identification of viral peptide fragments for vaccine development.鉴定用于疫苗开发的病毒肽片段。
Methods Mol Biol. 2009;515:261-74. doi: 10.1007/978-1-59745-559-6_18.
8
Random dissection to select for protein split sites and its application in protein fragment complementation.用于选择蛋白质裂解位点的随机切割及其在蛋白质片段互补中的应用。
Protein Sci. 2009 Feb;18(2):399-409. doi: 10.1002/pro.42.
9
Single mutations convert an outward K+ channel into an inward K+ channel.单个突变可将外向钾离子通道转变为内向钾离子通道。
Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):2871-6. doi: 10.1073/pnas.0712349105. Epub 2008 Feb 19.
10
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Nucleic Acids Res. 2006;34(18):5259-69. doi: 10.1093/nar/gkl548. Epub 2006 Sep 26.
Nature. 1994 Aug 4;370(6488):389-91. doi: 10.1038/370389a0.
4
Applied evolution. The progeny of sexual PCR.应用进化。有性PCR的后代。
Nature. 1994 Aug 4;370(6488):324-5. doi: 10.1038/370324a0.
5
DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution.通过随机片段化和重新组装进行DNA改组：用于分子进化的体外重组
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6
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Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites.盒式诱变：一种在特定位点产生多个突变的有效方法。
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B3(Fv)-PE38KDEL, a single-chain immunotoxin that causes complete regression of a human carcinoma in mice.B3(Fv)-PE38KDEL，一种能使小鼠体内的人类癌瘤完全消退的单链免疫毒素。
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