大肠杆菌30S核糖体蛋白与50S亚基之间的相互作用。
Interactions between 30s ribosomal proteins and 50s subunits of Escherichia coli.
作者信息
Jenness D, Bruce J, Schaup H W
出版信息
Nucleic Acids Res. 1976 Jan;3(1):1-10. doi: 10.1093/nar/3.1.1.
Abstract
The interaction between ribosomal proteins of the 30s subunit with intact 50s subunits was investigated. Experiments with mixtures of total 30s proteins indicated that several 30s proteins including protein s4 would form a stable complex with 50s subunits. Further work with pure s4 indicates that this protein binds stoichiometrically to the 50s subunits, probably through protein-nucleic acid interaction. The possible significance of this interaction is discussed.
摘要
研究了核糖体30S亚基的核糖体蛋白与完整50S亚基之间的相互作用。对30S总蛋白混合物进行的实验表明，包括蛋白s4在内的几种30S蛋白会与50S亚基形成稳定的复合物。对纯s4的进一步研究表明，该蛋白以化学计量方式与50S亚基结合，可能是通过蛋白质-核酸相互作用。讨论了这种相互作用的可能意义。
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本文引用的文献
1
Reconstitution of active ribosomes with cross-linked proteins.
Nat New Biol. 1973 May 2;243(122):15-7.
2
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
3
Stoichiometry of the 30S ribosomal proteins of Escherichia coli.
Biochemistry. 1971 Feb 2;10(3):517-24. doi: 10.1021/bi00779a026.
4
Correlation of 30S ribosomal proteins of Escherichia coli isolated in different laboratories.
Mol Gen Genet. 1971;111(4):327-33. doi: 10.1007/BF00569784.
5
Ribosomal proteins. XXVI. The number of specific protein binding sites on 16 s and 23 s RNA of Escherichia coli.
J Mol Biol. 1971 Dec 14;62(2):411-4. doi: 10.1016/0022-2836(71)90437-2.
6
Alteration of a 30S ribosomal protein accompanying the ram mutation in Escherichia coli.
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2263-7. doi: 10.1073/pnas.68.9.2263.
7
Chemical modification of the transfer RNA and polyuridylic acid binding sites of Escherichia coli 30 s ribosomal subunits.
J Mol Biol. 1971 Nov 14;61(3):669-79. doi: 10.1016/0022-2836(71)90071-4.
8
Molecular interactions of ribosomal components. II. Site-specific complex formation between 30S proteins and ribosomal RNA.
Mol Gen Genet. 1971;112(1):1-8. doi: 10.1007/BF00266926.
9
Reversion of a streptomycin-dependent strain of Escherichia coli.
Mol Gen Genet. 1970;109(4):356-69. doi: 10.1007/BF00267704.
10
Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.
Anal Biochem. 1970 Aug;36(2):401-12. doi: 10.1016/0003-2697(70)90376-3.
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