大肠杆菌16S核糖体RNA 3'端附近两个相邻的N6,N6-二甲基腺苷功能的研究。IV. 甲基对核糖体亚基相互作用的影响。
Studies on the function of two adjacent N6,N6-dimethyladenosines near the 3' end of 16S ribosomal RNA of Escherichia coli. IV. The effect of the methylgroups on ribosomal subunit interaction.
作者信息
Poldermans B, Bakker H, Van Knippenberg P H
出版信息
Nucleic Acids Res. 1980 Jan 11;8(1):143-51. doi: 10.1093/nar/8.1.143.
Abstract
The effect of the presence or absence of the methylgroups of the m2(6)Am2(6)A sequence near the 3' end of 16S rRNA of Escherichia coli on the interaction of the ribosomal subunits has been studied, using wild-type (methylated) and mutant (unmethylated) ribosomes. Subunit exchange experiments and competitive association experiments show a strong preference of the 50S subunit for association with methylated 30S subunits. The results indicate that the equilibrium constant of the reaction 70S in equilibrium with 30S + 50S is dependent on the methylgroups; mutant 30S.50S couples are less stable than wild-type 30S.50S couples. It is postulated that the methylgroups also stimulate the interaction between 30S subunits and initiation factor IF-3.
摘要
利用野生型（甲基化）和突变型（未甲基化）核糖体，研究了大肠杆菌16S rRNA 3'端附近m2(6)Am2(6)A序列甲基基团的存在与否对核糖体亚基相互作用的影响。亚基交换实验和竞争性结合实验表明，50S亚基强烈倾向于与甲基化的30S亚基结合。结果表明，70S与30S + 50S平衡反应的平衡常数取决于甲基基团；突变型30S.50S对不如野生型30S.50S对稳定。据推测，甲基基团还刺激30S亚基与起始因子IF-3之间的相互作用。
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本文引用的文献
1
Classification of methanogenic bacteria by 16S ribosomal RNA characterization.通过16S核糖体RNA特征对产甲烷菌进行分类。
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4537-41. doi: 10.1073/pnas.74.10.4537.
2
Inactivation of ribosomes by colicin E3 in vitro: Requirement for 50 S ribosomal subunits.大肠杆菌素E3在体外使核糖体失活：对50S核糖体亚基的需求
FEBS Lett. 1972 Apr 15;22(1):73-75. doi: 10.1016/0014-5793(72)80222-9.
3
Inactivation of ribosomes in vitro by colicin E 3 and its mechanism of action.大肠杆菌素E3在体外对核糖体的失活作用及其作用机制。
Proc Natl Acad Sci U S A. 1972 Mar;69(3):549-52. doi: 10.1073/pnas.69.3.549.
4
Mechanism of kasugamycin resistance in Escherichia coli.大肠杆菌中春雷霉素抗性的机制。
Nat New Biol. 1972 Jan 5;235(53):6-9. doi: 10.1038/newbio235006a0.
5
Change in methylation of 16S ribosomal RNA associated with mutation to kasugamycin resistance in Escherichia coli.与大肠杆菌对春雷霉素耐药性突变相关的16S核糖体RNA甲基化变化
Nat New Biol. 1971 Sep 1;233(35):12-4. doi: 10.1038/newbio233012a0.
6
Effects of temperature on the activity of cloacin DF13 and cloacin DF13-immunity protein.温度对杀癌素DF13及杀癌素DF13免疫蛋白活性的影响
Biochim Biophys Acta. 1975 Jul 14;399(1):213-6. doi: 10.1016/0304-4165(75)90227-5.
7
Kinetics of the reversible association of ribosomal subunits: stopped-flow studies of the rate law and of the effect of Mg2+.核糖体亚基可逆结合的动力学：速率定律及Mg2+效应的停流研究
J Mol Biol. 1975 Apr 25;93(4):499-15. doi: 10.1016/0022-2836(75)90242-9.
8
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10
New aspects of the IF3-ribosome interaction.
FEBS Lett. 1976 Feb 15;62(2):111-4. doi: 10.1016/0014-5793(76)80030-0.
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