Schnitzer J E, McIntosh D P, Dvorak A M, Liu J, Oh P
Department of Pathology, Harvard Medical School, Beth Israel Hospital, Boston, MA 02215, USA.
Science. 1995 Sep 8;269(5229):1435-9. doi: 10.1126/science.7660128.
In situ coating of the surface of endothelial cells in rat lung with cationic colloidal silica particles was used to separate caveolae from detergent-insoluble membranes rich in glycosyl phosphatidylinositol (GPI)-anchored proteins but devoid of caveolin. Immunogold electron microscopy showed that ganglioside GM1-enriched caveolae associated with an annular plasmalemmal domain enriched in GPI-anchored proteins. The purified caveolae contained molecular components required for regulated transport, including various lipid-anchored signaling molecules. Such specialized distinct microdomains may exist separately or together in the plasma membrane to organize signaling molecules and to process surface-bound ligands differentially.
用阳离子胶体二氧化硅颗粒原位包被大鼠肺内皮细胞表面，以从富含糖基磷脂酰肌醇（GPI）锚定蛋白但缺乏小窝蛋白的去污剂不溶性膜中分离小窝。免疫金电子显微镜显示，富含神经节苷脂GM1的小窝与富含GPI锚定蛋白的环形质膜结构域相关。纯化的小窝包含调节转运所需的分子成分，包括各种脂锚定信号分子。这种特殊的独特微结构域可能在质膜中单独或共同存在，以组织信号分子并以不同方式处理表面结合的配体。
