Shambesh M K, Craig P S, Gusbi A M, Echtuish E F, Wen H
Department of Biological Sciences, University of Salford, UK.
Trans R Soc Trop Med Hyg. 1995 May-Jun;89(3):276-9. doi: 10.1016/0035-9203(95)90538-3.
Two high molecular weight antigens with molecular masses approximately 100 kDa and 130 kDa were identified by immunoblotting camel hydatid cyst fluid, with 94% sensitivity in sera from surgically confirmed Libyan cystic echinococcosis cases. 40% of sera from surgically confirmed alveolar echinococcosis cases cross-reacted with the 100 and 130 kDa antigens, as did 5.3% of sera from human Taenia solium cysticercosis patients. No cross-reaction occurred with sera from human schistosomiasis mansoni or onchocerciasis patients. In addition, all sera from patients with non-hydatid space-occupying lesions (i.e. simple liver cysts, kidney cysts, lung tuberculosis, pulmonary carcinoma, pulmonary empyema, and lung abscess) were seronegative against the same antigens, as were control serum samples from healthy individuals. The 100 and 130 kDa antigens were strongly recognized by sera from cystic echinococcosis patients when camel or horse hydatid cyst fluid was used in immunoblotting but were only weakly recognized if sheep or human hydatid cyst fluid was used. Camel hydatid cyst fluid could be an important source of diagnostic antigens for human cystic echinococcosis in the Middle East endemic region.
通过免疫印迹法分析骆驼包虫囊肿液，鉴定出两种分子量约为100 kDa和130 kDa的高分子量抗原，在经手术确诊的利比亚囊型棘球蚴病患者血清中的敏感性为94%。在经手术确诊的泡型棘球蚴病患者血清中，40%与100 kDa和130 kDa抗原发生交叉反应，猪带绦虫囊尾蚴病患者血清中的这一比例为5.3%。曼氏血吸虫病或盘尾丝虫病患者的血清未发生交叉反应。此外，所有非包虫占位性病变患者（即单纯肝囊肿、肾囊肿、肺结核、肺癌、肺脓肿和肺脓肿）的血清对相同抗原均呈血清学阴性，健康个体的对照血清样本也是如此。当在免疫印迹中使用骆驼或马包虫囊肿液时，100 kDa和130 kDa抗原能被囊型棘球蚴病患者的血清强烈识别，但如果使用绵羊或人包虫囊肿液，则只能被微弱识别。骆驼包虫囊肿液可能是中东流行地区人类囊型棘球蚴病诊断抗原的重要来源。
