Rodríguez M, Penichet M L, Mouris A E, Labarta V, Luaces L L, Rubiera R, Cordovés C, Sánchez P A, Ramos E, Soto A
Mammalian Cell Genetics Division, Centro de Ingeniería Genética y Biotecnología, Havana 6, Cuba.
Vet Parasitol. 1995 Apr;57(4):339-49. doi: 10.1016/0304-4017(94)00678-6.
Current methods for the control of cattle tick Boophilus microplus infestations are not effective and the parasite remains a serious problem for the cattle industry in tropical and subtropical areas. Recently, we developed a vaccine against B. microplus employing a recombinant Bm86 (rBm86) antigen preparation (Gavac, Heber Biotec) and it was shown to induce a protective response in vaccinated animals under controlled conditions. Here we show that, under field conditions in grazing cattle, the vaccine is able to control B. microplus populations. Two parasite-free farms were employed for the study. In the first farm, animals were vaccinated with the recombinant vaccine, while, in the second, animals received a saline injection in adjuvant. After immunization, animals were artificially infected and the infestation rate was recorded. Over the 33 weeks of the experiment, the infestation rate was lower in the vaccinated group compared with the control group. At the end of the experiment it was necessary to use chemicals in the control farm after serious losses in production and animals.
目前控制微小牛蜱（Boophilus microplus）感染的方法效果不佳，这种寄生虫对于热带和亚热带地区的养牛业来说仍然是一个严重问题。最近，我们利用重组Bm86（rBm86）抗原制剂（Gavac，赫伯生物技术公司）研发了一种针对微小牛蜱的疫苗，并且已证明该疫苗在可控条件下能使接种动物产生保护性反应。在此我们表明，在放牧牛群的田间条件下，该疫苗能够控制微小牛蜱种群数量。本研究使用了两个无寄生虫的农场。在第一个农场，动物接种了重组疫苗，而在第二个农场，动物接受了含佐剂的生理盐水注射。免疫后，动物被人工感染并记录感染率。在实验的33周内，接种组的感染率低于对照组。在实验结束时，由于生产严重损失和动物死亡，对照组农场不得不使用化学药品。
