Canales Mario, Almazán Consuelo, Naranjo Victoria, Jongejan Frans, de la Fuente José
Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo s/n, Ciudad Real, Spain.
BMC Biotechnol. 2009 Mar 31;9:29. doi: 10.1186/1472-6750-9-29.
The cattle ticks, Boophilus spp., affect cattle production in tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. The recombinant B. microplus Bm86 protective antigen has been shown to protect cattle against tick infestations. Recently, the gene coding for B. annulatus Bm86 ortholog, Ba86, was cloned and the recombinant protein was secreted and purified from the yeast Pichia pastoris.
Recombinant Ba86 (Israel strain) was used to immunize cattle to test its efficacy for the control of B. annulatus (Mercedes, Texas, USA strain) and B. microplus (Susceptible, Mexico strain) infestations. Bm86 (Gavac and Mozambique strain) and adjuvant/saline were used as positive and negative controls, respectively. Vaccination with Ba86 reduced tick infestations (71% and 40%), weight (8% and 15%), oviposition (22% and 5%) and egg fertility (25% and 50%) for B. annulatus and B. microplus, respectively. The efficacy of both Ba86 and Bm86 was higher for B. annulatus than for B. microplus. The efficacy of Ba86 was higher for B. annulatus (83.0%) than for B. microplus (71.5%). The efficacy of Bm86 (Gavac; 85.2%) but not Bm86 (Mozambique strain; 70.4%) was higher than that of Ba86 (71.5%) on B. microplus. However, the efficacy of Bm86 (both Gavac and Mozambique strain; 99.6%) was higher than that of Ba86 (83.0%) on B. annulatus.
These experiments showed the efficacy of recombinant Ba86 for the control of B. annulatus and B. microplus infestations in cattle and suggested that physiological differences between B. microplus and B. annulatus and those encoded in the sequence of Bm86 orthologs may be responsible for the differences in susceptibility of these tick species to Bm86 vaccines.
牛蜱（Boophilus spp.）影响着世界热带和亚热带地区的养牛业。蜱疫苗是一种经济高效且环保的蜱虫防治替代方法。已证明重组微小牛蜱Bm86保护性抗原可保护牛免受蜱虫侵扰。最近，已克隆了环形牛蜱Bm86直系同源基因Ba86的编码基因，并从毕赤酵母中分泌和纯化了重组蛋白。
使用重组Ba86（以色列菌株）免疫牛，以测试其对环形牛蜱（美国得克萨斯州梅赛德斯菌株）和微小牛蜱（墨西哥易感菌株）侵扰的防治效果。分别使用Bm86（加瓦克和莫桑比克菌株）和佐剂/生理盐水作为阳性和阴性对照。用Ba86疫苗接种可分别减少环形牛蜱和微小牛蜱的蜱虫侵扰（71%和40%）、蜱虫重量（8%和15%）、产卵量（22%和5%）以及卵的孵化率（25%和50%）。Ba86和Bm86对环形牛蜱的防治效果均高于对微小牛蜱的防治效果。Ba86对环形牛蜱的防治效果（83.0%）高于对微小牛蜱的防治效果（71.5%）。Bm86（加瓦克；85.2%）而非Bm86（莫桑比克菌株；70.4%）对微小牛蜱的防治效果高于Ba86（71.5%）。然而，Bm86（加瓦克和莫桑比克菌株；99.6%）对环形牛蜱的防治效果高于Ba86（83.0%）。
这些实验表明重组Ba86对防治牛身上的环形牛蜱和微小牛蜱侵扰有效，并表明微小牛蜱和环形牛蜱之间的生理差异以及Bm86直系同源基因序列中编码的差异可能是这些蜱虫物种对Bm86疫苗易感性差异的原因。
