Tanaka S, Taniue A, Nagao N, Ohnoki S, Shibata H, Okubo Y, Yamaguchi H
Department of Research, Osaka Red Cross Blood Center, Japan.
Vox Sang. 1995;68(4):225-30. doi: 10.1111/j.1423-0410.1995.tb02577.x.
We developed an allele-specific polymerase chain reaction (ASPCR) method using originally designed primers to determine the genotype of the human platelet antigens (HPAs) 2, 3 and 4 in parallel. The results were compared with those obtained by PCR restriction fragment length polymorphism and the mixed passive hemagglutination test. Seventy-three individuals were tested and the ASPCR results were in good agreement with those determined by the other two methods. This method enables the genotyping of HPA-2, -3 and -4 in parallel without the use of platelets, platelet-specific alloantibodies or restriction enzymes.
我们开发了一种等位基因特异性聚合酶链反应（ASPCR）方法，使用最初设计的引物来同时确定人类血小板抗原（HPA）2、3和4的基因型。将结果与通过聚合酶链反应-限制性片段长度多态性和混合被动血凝试验获得的结果进行比较。对73名个体进行了检测，ASPCR结果与其他两种方法确定的结果高度一致。该方法无需使用血小板、血小板特异性同种抗体或限制性内切酶，即可同时对HPA-2、-3和-4进行基因分型。
