Li Y F, Zuo Y C, Shao L, Song D M, Ding G F, Fan S G
Department of Physiology, Beijing Medical University.
Yao Xue Xue Bao. 1995;30(5):395-9.
Our previous work showed that a suppressive factor (a protein with large molecular weight) in serum was induced by restraint stress in mice and rats, which suppressed Con A induced lymphocyte proliferation. It was also found that the generation of serum suppressive factor was under control of the central nervous system. Our further study showed that intracerebroventricular (icv) injection of interleukin 1 receptor antagonist (IL-1Ra) antagonised the generation of serum suppressive factor induced by restraint stress and icv injection of interleukin-1 beta (IL-1 beta) increased the generation of the suppressive factor. Our experiment also showed that the serum suppressive factor induced by restraint stress was first made in lymph tissue and then released into blood. The present work was designed to investigate the role of IL-1 in the brain in generation of the suppressive factor in lymph node in mice. Icv injection of IL-1 beta (1 pg/mouse) was shown to significantly increase the generation of the suppressive factor in lymph node. Icv injection of IL-1Ra, however, antagonised generation of the suppressive factor. In mice without restraint stress, both the suppressive factor in serum and in lymph node were found to be induced in dose-dependent manner by icv injection of IL-1 beta. Taken together, these results suggest that IL-1 beta in brain played a very important role in generation of the suppressive factor in lymph node. The positive correlation between the suppressive action of lymph node and of serum added to the evidence that lymph tissue is probably the source of the serum suppressive factor.
我们之前的研究表明，小鼠和大鼠在束缚应激下血清中会诱导产生一种抑制因子（一种大分子蛋白质），该因子可抑制刀豆蛋白A诱导的淋巴细胞增殖。还发现血清抑制因子的产生受中枢神经系统的控制。我们进一步的研究表明，脑室内注射白细胞介素1受体拮抗剂（IL-1Ra）可拮抗束缚应激诱导的血清抑制因子的产生，而脑室内注射白细胞介素-1β（IL-1β）则会增加抑制因子的产生。我们的实验还表明，束缚应激诱导的血清抑制因子首先在淋巴组织中产生，然后释放到血液中。本研究旨在探讨脑中的IL-1在小鼠淋巴结中抑制因子产生过程中的作用。结果显示，脑室内注射IL-1β（1 pg/只小鼠）可显著增加淋巴结中抑制因子的产生。然而，脑室内注射IL-1Ra可拮抗抑制因子的产生。在无束缚应激的小鼠中，脑室内注射IL-1β可剂量依赖性地诱导血清和淋巴结中的抑制因子产生。综上所述，这些结果表明脑中的IL-1β在淋巴结中抑制因子的产生中起非常重要的作用。淋巴结与血清的抑制作用之间的正相关关系进一步证明淋巴组织可能是血清抑制因子的来源。
