[辛二酸海绵原甲藻素对豚鼠离体工作心脏血流动力学及心肌缺血再灌注损伤的影响]

[Effect of sodium suberogorgin on hemodynamics and myocardial ischemic-reperfused injury in isolated guinea pig working hearts].

作者信息

Xu S B, Peng W D, Hu Y T

机构信息

Department of Biology, Zhongshan University, Guangzhou, China.

出版信息

Zhongguo Yao Li Xue Bao. 1995 May;16(3):285-8.

PMID:7660830

Abstract

AIM

To study the actions of sodium suberogorgin (Sub) on normal- and myocardial ischemic-reperfused-working hearts.

METHODS

The isolated guinea pig working hearts were perfused with edetic acid (0.2 mumol.L(-1))-Krebs-Henseleit solution by left pulmonary vein at 37 +/- 0.5 degrees C. The perfusing pressure of left atrium and the afterload of left ventricle were maintained at 1.0 and 7.2 kPa, respectively. During 50-min low-flow global ischemia, coronary flow was maintained at 4.7 +/- 0.2% of normal working hearts via retrograding perfusion. Reperfusion lasted 35 min following ischemia.

RESULTS

Before ischemia, the aortic pressure, left ventricular systolic pressure, the maximal rate of left ventricular pressure change (+ dp/dtmax and -dp/dtmax), cardiac output, stroke volume, left ventricular end-diastolic pressure, and heart rate of working hearts treated with Sub 10 mumol.L(-1) for 10 min were increased by 14%, 17%, 17%, 22%, 15%, 32%, -200%, and -11%, respectively. These parameters were reduced when Sub was increased to 50 mumol.L(-1). Reperfusion aggravated the myocardial damages induced by ischemia. Sub 10 mumol.L(-1) used before and during low-flow ischemia completely restored all above parameters except heart rate to the level of preischemia. When Sub was increased to 50 mumol.L(-1), only an attenuation of myocardial damage was observed.

CONCLUSION

Sub obviously improved the ischemic-reperfused injury in isolated working hearts.

摘要

目的

研究辛二酸二钠（Sub）对正常及心肌缺血再灌注工作心脏的作用。

方法

将豚鼠离体工作心脏于37±0.5℃经左肺静脉用乙二胺四乙酸（0.2μmol·L⁻¹）- Krebs-Henseleit溶液灌注。左心房灌注压和左心室后负荷分别维持在1.0和7.2 kPa。在50分钟的低流量全心缺血期间，通过逆行灌注使冠状动脉流量维持在正常工作心脏的4.7±0.2%。缺血后再灌注持续35分钟。

结果

缺血前，用10μmol·L⁻¹ Sub处理10分钟的工作心脏的主动脉压、左心室收缩压、左心室压力变化最大速率（+dp/dtmax和 -dp/dtmax）、心输出量、每搏输出量、左心室舒张末期压力和心率分别增加了14%、17%、17%、22%、15%、32%、-200%和 -11%。当Sub增加到50μmol·L⁻¹时，这些参数降低。再灌注加重了缺血诱导的心肌损伤。在低流量缺血前及缺血期间使用10μmol·L⁻¹ Sub可使除心率外的所有上述参数完全恢复到缺血前水平。当Sub增加到50μmol·L⁻¹时，仅观察到心肌损伤减轻。