Xu S B, Peng W D, Hu Y T
Department of Biology, Zhongshan University, Guangzhou, China.
Zhongguo Yao Li Xue Bao. 1995 May;16(3):285-8.
To study the actions of sodium suberogorgin (Sub) on normal- and myocardial ischemic-reperfused-working hearts.
The isolated guinea pig working hearts were perfused with edetic acid (0.2 mumol.L(-1))-Krebs-Henseleit solution by left pulmonary vein at 37 +/- 0.5 degrees C. The perfusing pressure of left atrium and the afterload of left ventricle were maintained at 1.0 and 7.2 kPa, respectively. During 50-min low-flow global ischemia, coronary flow was maintained at 4.7 +/- 0.2% of normal working hearts via retrograding perfusion. Reperfusion lasted 35 min following ischemia.
Before ischemia, the aortic pressure, left ventricular systolic pressure, the maximal rate of left ventricular pressure change (+ dp/dtmax and -dp/dtmax), cardiac output, stroke volume, left ventricular end-diastolic pressure, and heart rate of working hearts treated with Sub 10 mumol.L(-1) for 10 min were increased by 14%, 17%, 17%, 22%, 15%, 32%, -200%, and -11%, respectively. These parameters were reduced when Sub was increased to 50 mumol.L(-1). Reperfusion aggravated the myocardial damages induced by ischemia. Sub 10 mumol.L(-1) used before and during low-flow ischemia completely restored all above parameters except heart rate to the level of preischemia. When Sub was increased to 50 mumol.L(-1), only an attenuation of myocardial damage was observed.
Sub obviously improved the ischemic-reperfused injury in isolated working hearts.
研究辛二酸二钠（Sub）对正常及心肌缺血再灌注工作心脏的作用。
将豚鼠离体工作心脏于37±0.5℃经左肺静脉用乙二胺四乙酸（0.2μmol·L⁻¹）- Krebs-Henseleit溶液灌注。左心房灌注压和左心室后负荷分别维持在1.0和7.2 kPa。在50分钟的低流量全心缺血期间，通过逆行灌注使冠状动脉流量维持在正常工作心脏的4.7±0.2%。缺血后再灌注持续35分钟。
缺血前，用10μmol·L⁻¹ Sub处理10分钟的工作心脏的主动脉压、左心室收缩压、左心室压力变化最大速率（+dp/dtmax和 -dp/dtmax）、心输出量、每搏输出量、左心室舒张末期压力和心率分别增加了14%、17%、17%、22%、15%、32%、-200%和 -11%。当Sub增加到50μmol·L⁻¹时，这些参数降低。再灌注加重了缺血诱导的心肌损伤。在低流量缺血前及缺血期间使用10μmol·L⁻¹ Sub可使除心率外的所有上述参数完全恢复到缺血前水平。当Sub增加到50μmol·L⁻¹时，仅观察到心肌损伤减轻。
Sub明显改善了离体工作心脏的缺血再灌注损伤。
